1. Relevance of response to recombinant allergens of Aspergillus fumigatus in patients with chronic or acute respiratory diseases Barocci Fiorella (1), De Bernardi Giorgio (2), Lodato Antonio (1) (1) Immunotrasfusion Center Hospital of Rho – Company Hospital G. Salvini Garbagnate Milanese, (2) Department of Respiratory Medicine - Santa Corona Hospital Garbagnate Milanese, Italy BACKGROUND This study was conducted in adults hospitalized in the Department of Respiratory Medicine suffering from chronic obstructive pulmonary disease - COPD, from pneumonia, from severe chronic asthma, from allergic bronchopulmonary aspergillosis - ABPA, or from severe chronic asthma. serological for diagnosis of aspergillus allergy. OBJECTIVES Our objective was to improve the use of diagnostic serological for diagnosis of aspergillus allergy. SUMMARY Adult patients hospitalized for different chronic obstructive pulmonary diseases were serologically investigated to diagnose which molecular components can be related to the allergic aspergillosis. The use of serological routine diagnostic wanted to point out which recombinant should be considered as a "marker" when the allergen native of A. fumigatus was positive. In selected patients without distinction of pathology, Asp f 1 was found to be the most frequent positive component. By the way, we should remark that sometimes we have other molecular recombinants resulting positive. RESULTS The results obtained show that only 6 patients were positive, from 0.64 to 0.79 KUA/l and only one with 31.1 KUA/l, to the native allergen of A. fumigates, but they were negative to all the recombinant. Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens and it’s both a primary and opportunistic pathogen causing allergic and invasive aspergillosis in humans and animalls. A. fumigatus can be responsible of colonization in the lung area starting different diseases, the severity of which depends on the host’s immune response: on the complexity of the antigenic structure of the A. fumigates, on the varying responses of the different hosts and on clinical condition such as hypersensitivity, pneumonitis, allergic rhinitis, IgE-mediated asthma, allergic bronchopulmonary aspergillosis (ABPA), aspergilloma and invasive aspergillosis in immune-compromised patients and in subject with cistis fibrosis. CONCLUSIONS REFERENCES MATERIALS & METHODS We identified 30 patients that were positive by dosing for IgE antibodies to the native allergen of the A. fumigatus with the test system ImmunoCap of Phadia S.r.l. of Thermo Fisher Scientific. As regards negative control, we identified 10 patients that were negative by dosing IgE antibodies to the native allergen and they were negative to all the different components available. This control was the demonstration of the sensitivity and specificity of the test with the use of M3 native allergen of A. fumigatus. From these initial evaluations we can highlight the greater frequency of positivity of patients at component Asp f 1. The difference of the results may depend on the patient’s pathology and/or their consequent different immunological response to the various molecular components. Without distinction to pathology the patients positive to the allergen native of A. fumigatus should be evaluated with all the recombinant available with the aim to exclude false positivity. -Latgè JP. The pathobiology of Aspergillus fumigatus. Trends Microbiol. 2001;9:382-9. -Sarfati J, Monod M, Recco P, Sulahian A, Pinel C, Candolfi E, Fontaine T, Debeaupuis JP, Tabouret M, Latgé JP. Recombinant antigens as diagnostic markers for aspergillosis. Diagn Microbiol Infect Dis 2006;55(4):279-91. - Barocci F, De Bernardi G. Antibody response to recombinant of A. fumigatus in patients with chronic obstructive pulmonary disease, EAACI 2014 Poster session 64 n. 1430. Tips for Inserting Graphs or Images Note: Skip the following procedure if your graphs were created in PowerPoint®, Illustrator (eps file) or Excel. Image checking procedure: After you insert the image (72 dpi screen resolution) and resize* to fit, right click on it and select Format Picture. When the pop-up window comes up, click on size and check the scale. The image will print better if its width and height scale is at 25% or lower (20% or 10%, etc.) If the scale of the image is higher than 25%, try to replace it with a larger size (more dpi, e.g. 300dpi) image if possible. (Note: This should not be done by manually stretching the image to a larger size.) 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This is only a reminder.) M3 positive / all the recombinant negative Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens and it’s both a primary and opportunistic pathogen causing allergic and invasive aspergillosis in humans and animals. All the different components available, Asp f 1, Asp f 2, Asp f 3, Asp f 4, Asp f 6, were evaluated in the patients positive to the allergen native of A. fumigatus without distinction of pathology. Some patients had allergic predisposition being positive to other allergens such as mold and mites. Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens and it’s both a primary and opportunistic pathogen causing allergic and invasive aspergillosis in humans and animals. Aspergillus fumigates can lead to begin colonisation of the lung by several diseases, the severity of which depends on the host’s immune response: as a result of the complexity in the antigenic structure of the A. fumigates, the varying responses of the different hosts and for clinical condition such as hypersensitivity, pneumonitis, allergic rhinitis, IgE-mediated asthma, allergic bronchopulmonary aspergillosis (ABPA), aspergilloma and invasive aspergillosis in immune-compromised patients and in individuals with cistis fibrosis. In relation to this presentation, I declare that there are no conflicts of interest