1. Overview of Instruments/Services at the Analytical Instrumentation Core Matthew Au Array Service Manager December 1 st, 2015

2. Overview of AIC Instruments

3. Services Offered 1.Bio-Rad Supply Center 2.qRT-PCR Array Service 3.Lipid Analysis Service ▫ Fatty Acids/Monosaccharides 4.Protein/Cytokine Array Service ▫ Luminex Platform ▫ Multiplexing

4. Protein/Cytokine Arrays as an Extension of qRT-PCR Logical extension of PCR Arrays that can verify that changes at the mRNA level (gene expression) correspond to changes at the protein level Can also be used to identify the presence and amount of various proteins/cytokines in a sample Viewed as an alternative to running ELISAs

5. Brief Overview of the Workflow 1.Researchers will submit their samples ▫ Plasma, serum, cell supernatants, etc. 2.Purchase kits from various vendors ▫ EMD Millipore, R&D Systems, Bio-Rad, etc. 3.Kits contain magnetic beads that already have capture antibodies and are fluorescently coated Luminex Magpix Instrument

6. Miniaturization of Protein Arrays Incorporated Curiox Biosystems’ DropArray Technology Typically run the assays on a regular 96-well microtiter plate using handheld magnet (bottom left) Conversion has allowed a 5-fold reduction in the amount sample volume and reagents required Conventional MTP and Handheld Magnet Wall-Less Plate and Automated Plate Washer

7. Advantages of Miniaturization Reduces total assay costs 60-80%

8. Sample Data MILLIPLEX Analyst Detail Report Summary Analyte Sample IFN- Gamma IL-6IL-10IL-12p70TNF-Alpha pg/ml QC1186.92164.66160.91171.45153.37 QC2940.9699.39836.06804.14902.4 174470.66810.25319.54699.4724.65 176152852272318414532.32 236741.791056622.11846.558 204152.861676473.97800.18<3.10↓ 2661143606.9814461708<3.10↓ 298175.65644.84189.53265.83<3.10↓ 2105.6837.1911.518.66<3.10↓ 174B>15905↑34271744694.749.06 176B>15905↑89053354233257.02 236B1454370282723663.6756.97 204B>15905↑32221576304.4943.94 266B1199910171953794.4927.99 298B>15905↑3262207385.4176.13 210B13691509.01553.76<3.15↓23.14 Mouse 5-Plex Kit from EMD Millipore

9. Cancer Cardiovascular Cell Signaling Cellular Metabolism Immunology Metabolism & Endocrinology Neuroscience Stem Cells Toxicity Custom Different Kits Available

10. Contact Us! Array Service Manager Matthew Au mau115@bu.edu 617-414-2964 Central Lab Director of AIC Lynn Lingyi Deng, Ph.D., lynndeng@bu.edu 617-638-6920 office