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EUK 1209 CHLO02 DINO B PELA01 12.06.0002.08.0103.22.01 12.06.00 TetraselmisAlexandriumEucampiaChrys ochomulina BolidomonadAchnanthesAlteromonas DNA.

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Presentation on theme: "EUK 1209 CHLO02 DINO B PELA01 12.06.0002.08.0103.22.01 12.06.00 TetraselmisAlexandriumEucampiaChrys ochomulina BolidomonadAchnanthesAlteromonas DNA."— Presentation transcript:

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3 EUK 1209 CHLO02 DINO B PELA TetraselmisAlexandriumEucampiaChrys ochomulina BolidomonadAchnanthesAlteromonas DNA PCR Controls Pelagomonad BOLIDO03 PRYM02

4 Probe Progress

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6 P.salina Storeatu Rhinomon Rhodomar RhoM1480 Rhodoabb Psulcata Crypt.ph Guillard CCMP325 _ Geminige Tamphiox Chroosp2 Komma ChrM1481 ChrM1318 ChrM1312 Hrufesce Hbrunnes Hviresce CryM0739 CRYM117 CRypKo6 CrypM42 ChilomoM CryM1094 Goniomon Crypt.sp G.wittro C.parado Cyanopty G.nostoc F_distic Crcd.coh 0.01 substitutions/site

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9 asterion Asterkar Rhapbelg Fragcrot Fragvire SYneulna Fragisla fragstri Frag.spp Syedrasp Thaleleg Tnitschi Asterfor Diatoma Rhabdone Staurcon Pinnular cfCalone Striasp. Surirel Undatell PsNpuMG PsNpuMC PsNpuMP PseNmult PsNpuPB PseNpung PsNausMB Fragopsi Lyrella Gomparv2 Cymbcymb Encyonem Anomoene Achnlanc Cocconei Amphopro Campdisc Ampmont2 Amphocap Amphipro EUnotDGM Rossia Navsubm2 Nav.min2 Sellapup Phaeotri Eunotia Bacpaxil Achnasp1 Achnabon PseudoGo PseuGomp Navicula PFOLSYMB PBALSYMB Pleurosp NitzCFfr N.apicul Cylclost Cyclopho Microtab Gramtocn Lamprisc BID.P782 Cymatbel Papelega eucampia Phaeosce chaetros Chaetdid Chaetosp Pleulaev Odontsin Belleroc Lithodes Streptam Ditylbri Skel1009 Skelsubs skelcosk SkelcosP SkelcosA SkpseMED Skelmenz SKelmen2 Thalpseu Thalsp Thalprof Thaleccn Thalrotu Planksol Cyclmene cyc men Detoncon Thalfluv Poroglac Lauderia Leptodan Leptomin Stephbro Aulacdis Aulacamb Aulanyan Aulasuba Aulislan AULASKV2 AulabAB8 AulabAB2 Melovars Melvar3 Probosci Coscradi Actinopt Actinoc2 Actinocy Rhizoset RHizose1 Guinflac RHSETGR2 Rhimbric Guindeli COHISTRI Corecrio coreiner Flag46SE Flag94SC Flag31SE PARALIA DICTYOCH RHIZOCHR PSEUDOPE APEDINE Pelasubv Pelagosu CCMP1395 Pelagomd CCMP1145 Aureocan par.cl7 par.cl35 Pela1410 AureLagu Pulvinar Sarcinom N.salina Nannsal2 Nannosp1 Nanngran Nannosp2 Nannocul Monodops Vischeri Eustigma Pseuchar Flag11SA Flag77SC O_danica M.striat M_papill S.espino C_chromo Chrysosp Girauste F_distic Saccopol Chordato Desmarli Sporocom Alarmarg Macrocys Scytolom Ectosili Heterosi Chattone Vacuolar L_gigant P_megasp A_bisexu Developa TKRRE UPRRE Lbyr.min CFRRN16L AF AF changes Bolidomonads Pelagophytes Eustigmatophytes Silicoflagellates Diatoms

10 Array of four A. tamarense and one A. lusitanicum strain from different geographic regions hybridised with species- and strain-specific FITC-labelled probes. In situ Hybridisation

11 DINO01 AOST02AOST01 In situ Hybridisation of Alexandrium Species with Specific rRNA Probes

12 <15 <10 >70 >50 >40 <5 Dino01 Aost01 Atam01 Atna01 Station 9 Station 14 Probes A. tamarense siteA. ostenfeldii site

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