1. BME 301: Biomedical Sensors Lecture Note 3: Bioelectric Potentials and Biopotential Electrodes BME 301 Lecture Note 3 - Ali Işın 2013 1

2. Bioelectric potentials 2BME 301 Lecture Note 3 - Ali Işın 2013

3. RESTING POTENTIAL-BASIC CONCEPT Cell membranes are typically permeable to only a subset of ionic species like pottasium(K+),Chloride(Cl-) & effectively blocks the entry of sodium(Na+) ions. The various ions seeks a balance between inside & outside the cell according to concentration & electric charge. Two effects result from inability of Na+ ions to penetrate membrane- Concentration of Na+ ions inside cell is much lower than outside. Hence,outside of cell becomes more positive than inside. In an attempt to to balance electric charge,additional K+ ions enters the cell,causing higher concentration of K+ ion inside the cell. Charge balance can never be reached. Equilibrium is reached with a potential difference across the membrane, negative on inside and positive on outside called Resting Potential. 3 Polarized Cell during RP BME 301 Lecture Note 3 - Ali Işın 2013

4. RESTING POTENTIAL IN NERVE CELL  A nerve cell has an electrical potential, or voltage, across its cell membrane of approximately 70 millivolts (mV). This means that this tiny cell produces a voltage roughly equal to 1/20th that of a flashlight battery (1.5 volts).  The potential is produced by the actions of a cell membrane pump, powered by the energy of ATP.  As shown in Figure, this membrane protein forces sodium ions (Na+) out of the cell, and pumps potassium ions (K+) in. As a result of this active transport, the cytoplasm of the neuron contains more K+ ions and fewer Na+ ions than the surrounding medium. However, the neuron cell membrane is much leakier to K+ than it is to Na+. As a result, K+ ions leak out of the cell to produce a negative charge on the inside of the membrane.  This charge difference is known as the Resting Potential of the neuron. The neuron is not actually "resting" because it must produce a constant supply of ATP to fuel active transport. 4BME 301 Lecture Note 3 - Ali Işın 2013

5. RESTING POTENTIAL PROPOGATION 5 OUTSIDE INSIDE K + = Potassium; Na + = Sodium; Cl - = Chloride; Pr - = proteins Na + K+K+ K+K+ Force of Diffusion Electrostatic Force + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - Cl - Force of Diffusion Cl - Electrostatic Force Pr - Closed channel open channel open channel no channel 3Na/2K pump - 65 mV BME 301 Lecture Note 3 - Ali Işın 2013

6. ACTION POTENTIAL-BASIC CONCEPT  When section of cell membrane is excited by some form of externally applied energy, membrane characteristics changes & begins to allow some sodium ions to enter.  This movement of Na+ ions constitutes an ionic current that further reduces the barrier of the membrane to Na+ ions.  Result-Avalanche effect, Na+ ions rush into the cell to balance with the ions outside.  At the same time K+ ions which were in higher concentration inside the cell during resting state, try to leave the cell but are unable to move as rapidly as Na+ ions.  As a result the cell has slightly positive potential on inside due to imbalance of K+ ions.  This potential is called as Action Potential. 6 Depolarized cell during AP BME 301 Lecture Note 3 - Ali Işın 2013

7. WAVEFORM SHOWING DEPOLARIZATION & REPOLARIZATION IN ACTION POTENTIAL  The cell that displays an action Potential is said to be depolarized;  The process of changing from resting state to action potential is called Depolarization.  Once the rush of Na+ ions through the cell membrane has stopped, the membrane reverts back to its original condition wherein the passage of Na+ ions from outside to inside is blocked  This process is called Repolarization. 7BME 301 Lecture Note 3 - Ali Işın 2013

8. ACTION POTENTIAL PROPOGATION  It “travels” down the axon  Actually, it does not move. Rather the potential change resulting from Na+ influx disperses to the next voltage-gated channel, triggering another action potential there. 8BME 301 Lecture Note 3 - Ali Işın 2013

9. PROPOGATION OF POTENTIALS IN NERVE IMPULSE  The Moving Impulse An impulse begins when a neuron is stimulated by another neuron or by the environment. Once it begins, the impulse travels rapidly down the axon away from the cell body and towards the axon terminals.  As Figure shows, an impulse is a sudden reversal of the membrane potential. What causes the reversal? The neuron membrane contains thousands of protein channels or gates, that allow ions to pass through. Generally, these gates are closed. At the leading edge of an impulse, however, sodium gates open, allowing positively charged Na+ ions to flow inside. The inside of the membrane temporarily becomes more positive than the outside, reversing the resting potential. This reversal of charges is called an Action Potential. As the action potential, potassium gates open, allowing positively charged K+ ions to flow out. This restores the Resting Potential so that the neuron is once again negatively charged on the inside of the cell membrane and positively charged on the outside. 9BME 301 Lecture Note 3 - Ali Işın 2013

10.  A nerve impulse is self-propagating. That is, an impulse at any point on the membrane causes an impulse at the next point along the membrane. We might compare the flow of an impulse to the fall of a row of dominoes. As each domino falls, it causes its neighbour to fall. Then, as the impulse passes, the dominoes set themselves up again, ready for another Action Potential. BME 301 Lecture Note 3 - Ali Işın 201310

11. Resting and action potentials  The resting potential is the result of an unequal distribution of ions across the membrane.  The resting potential is sensitive to ions in proportion to their ability to permeate the membrane. 11BME 301 Lecture Note 3 - Ali Işın 2013

12. Resting potentials  Forget the membrane and consider what factors determine the movement of ions in solution.  Aqueous diffusion and  Electrophoretic movement 12BME 301 Lecture Note 3 - Ali Işın 2013

13. 0 mV Resting potentials 13BME 301 Lecture Note 3 - Ali Işın 2013

14. 0 mV Resting potentials 14BME 301 Lecture Note 3 - Ali Işın 2013

15. -80 mV Resting potentials 15BME 301 Lecture Note 3 - Ali Işın 2013

16. + + + + + + + - - - - - - -80 mV Resting potentials 16BME 301 Lecture Note 3 - Ali Işın 2013

17. + + + + + + + - - - - - - -80 mV [K+] = 2.5 [Na+] = 125 [Cl-] = 130 A- [K+] = 135 [Na+] = 7 [Cl-] = 11 A- Resting potentials 17BME 301 Lecture Note 3 - Ali Işın 2013

18. Resting potentials Resting membrane potential is independent of external Na+ concentration 18BME 301 Lecture Note 3 - Ali Işın 2013

19. Resting potentials Resting membrane potential strongly depends upon the external K+ concentration 19BME 301 Lecture Note 3 - Ali Işın 2013

20. Summary  The membrane conducts ions very poorly and allows the separation of ionic species.  This results in a potential difference between the outside and the inside of the membrane.  The magnitude of the resting potential is determined by the selective permeability of the membrane to ionic species.  We can quantify the magnitude of the resting potential by considering both the diffusive and electrophoretic properties.  In order to understand the time dependence and individual contributions of ionic species to the membrane potential it is convenient to use an electrical equivalent circuit. 20BME 301 Lecture Note 3 - Ali Işın 2013

21. Resting Membrane Potential Membrane outside inside Na + Cl - K+K+ K+K+ A-A- +++++++++++ ----------- +++++++++++ ----------- 21BME 301 Lecture Note 3 - Ali Işın 2013

22. Membrane is polarized  more negative particles in than out  Bioelectric Potential  like a battery  Potential for ion movement  current ~ 22BME 301 Lecture Note 3 - Ali Işın 2013

23. INSIDE POS NEG Bioelectric Potential OUTSIDE 23BME 301 Lecture Note 3 - Ali Işın 2013

24. Biopotentials  ECG  electrocardiogrphy  EEG  electroencephalography  EMG  electromyography  ERG  electroretinograpy  EOG…  electrooculography 24BME 301 Lecture Note 3 - Ali Işın 2013

25. Frequencies of Biopotentials 25BME 301 Lecture Note 3 - Ali Işın 2013

26. Electrocardiogram (ECG) 26BME 301 Lecture Note 3 - Ali Işın 2013

27. Recording System EEG  EEG recording is done using a standard lead system called 10-20 system  Recall dipole concept to identify source of brain activity 27 BME 301 Lecture Note 3 - Ali Işın 2013

28. Electromyogram (EMG)  Measures muscle activity  Recordintramuscularly through needle electrodes  Record surface EMG using electrodes on biceps,triceps…  Use in muscular disorders,muscle based prosthesis – prosthetic arm, leg 28BME 301 Lecture Note 3 - Ali Işın 2013

29. Electroretinogram (ERG)  Biopotential of the eye (retina)  Indicator of retinal diseases such as retinal degenration, macular degernation  Invasive recording 29BME 301 Lecture Note 3 - Ali Işın 2013

30. KINDS OF ELECTRODES Electrodes 30BME 301 Lecture Note 3 - Ali Işın 2013

31. Eectrodes 31BME 301 Lecture Note 3 - Ali Işın 2013

32. Figure A disposable surface electrode. A typical surface electrode used for ECG recording is made of Ag/AgCl. The electrodes are attached to the patients’ skin and can be easily removed. Eectrodes 32BME 301 Lecture Note 3 - Ali Işın 2013

33. Biopotential Electrodes 33BME 301 Lecture Note 3 - Ali Işın 2013

34. The current crosses it from left to right. The electrode consists of metallic atoms C. The electrolyte is an aqueous solution containing cations of the electrode metal C+ and anions A–. Electrode–electrolyte interface where n is the valence of C and m is valence of A 34BME 301 Lecture Note 3 - Ali Işın 2013

35. 35BME 301 Lecture Note 3 - Ali Işın 2013

36. V p = total patential, or polarization potential, of the electrode E 0 = half-cell potential V r = ohmic overpotential V c = concentration overpotential V a = activation overpotential 36BME 301 Lecture Note 3 - Ali Işın 2013

37. 37BME 301 Lecture Note 3 - Ali Işın 2013

38. A silver/silver chloride electrode, shown in cross section 38BME 301 Lecture Note 3 - Ali Işın 2013

39. 1.73  10 –10  142.3 = 2.46  10 –8 g 39BME 301 Lecture Note 3 - Ali Işın 2013

40. Sintered Ag/AgCI electrode 40BME 301 Lecture Note 3 - Ali Işın 2013

41. E hc is the half-cell potential, R d and C d make up the impedance associated with the electrode-electrolyte interface and polarization effects, Rs is the series resistance associated with interface effects and due to resistance in the electrolyte. Equivalent circuit for a biopotential electrode in contact with an electrolyte 41BME 301 Lecture Note 3 - Ali Işın 2013

42. The electrode area is 0.25 cm2. Numbers attached to curves indicate number of mA  s for each deposit. (From L. A. Geddes, L. E. Baker, and A. G. Moore, “Optimum Electrolytic Chloriding of Silver Electrodes,” Medical and Biological Engineering, 1969, 7, pp. 49–56.) Impedance as a function of frequency for Ag electrodes coated with an electrolytically deposited AgCl layer 42BME 301 Lecture Note 3 - Ali Işın 2013

43. Experimentally determined magnitude of impedance as a function of frequency for electrodes 43BME 301 Lecture Note 3 - Ali Işın 2013

44. Example 44 We want to develop an electrical model for a specific biopotential electrode studies in the laboratory. The electrode is characterized by placing it in a physiological saline bath in the laboratory, along with an Ag/AgCl electrode having a much greater surface area and a known half-cell potential of 0.233 V. The dc voltage between the two electrodes is measured with a very-high-impedance voltmeter and found to be 0.572 V with the test electrode negative The magnitude of the impedance between two electrodes is measured as a function of frequency at very low currents; it is found to be that given in Figure in slide 12. From these data, determine a circuit model for the electrode. BME 301 Lecture Note 3 - Ali Işın 2013

45. Solution 45 Half cell potential of the test electrode E hc = 0.223 V – 0.572 = -0339 V At frequencies greater than 20 kHz C d is short circuit. Thus R s = 500 Ω = 0.5 kΩ, At frequencies less than 50 Hz C d is open circuit. Thus R s + R d = 30 kΩ. Thus R d = 30 kΩ - R s = 29.5 kΩ Corner frequency is 100 Hz. Thus C d = 1/(2πf R d ) = 1/(2π100×29500) = 5.3×10 -8 F = 0.53×10 -9 F = 0.53 nF = -0339 V = 500 Ω = 29.5 kΩ = 0.53 nF BME 301 Lecture Note 3 - Ali Işın 2013

46. Magnified section of skin, showing the various layers 46BME 301 Lecture Note 3 - Ali Işın 2013

47. Each circuit element on the right is at approximately the same level at which the physical process that it represents would be in the left- hand diagram. A body-surface electrode is placed against skin, showing the total electrical equivalent circuit obtained in this situation 47BME 301 Lecture Note 3 - Ali Işın 2013

48. (a)Metal-plate electrode used for application to limbs, (b)Metal-disk electrode applied with surgical tape, (c)Disposable foam-pad electrodes, often used with electrocardiographic monitoring apparatus. Body-surface biopotential electrodes 48BME 301 Lecture Note 3 - Ali Işın 2013

49. fig_05_10 A metallic suction electrode is often used as a precordial electrode on clinical electrocardiographs. A metallic suction electrode 49BME 301 Lecture Note 3 - Ali Işın 2013

50. (a)Recessed electrode with top-hat structure, (b)Cross-sectional view of the electrode in (a), (c)Cross-sectional view of a disposable recessed electrode of the same general structure shown in figure (c) in slide 17. The recess in this electrode is formed from an open foam disk, saturated with electrolyte gel and placed over the metal electrode. Examples of floating metal body-surface electrodes 50BME 301 Lecture Note 3 - Ali Işın 2013

51. (a)Carbon-filled silicone rubber electrode, (b)Flexible thin-film neonatal electrode (after Neuman, 1973). (c)Cross-sectional view of the thin-film electrode in (b). 51 Flexible body-surface electrodes BME 301 Lecture Note 3 - Ali Işın 2013

52. (a)Insulated needle electrode, (b)Coaxial needle electrode, (c)Bipolar coaxial electrode, (d)Fine-wire electrode connected to hypodermic needle, before being inserted, (e)Cross-sectional view of skin and muscle, showing fine-wire electrode in place, (f)Cross-sectional view of skin and muscle, showing coiled fine-wire electrode in place. 52 Needle and wire electrodes for percutaneous measurement of biopotentials BME 301 Lecture Note 3 - Ali Işın 2013

53. (a)Suction electrode, (b)Cross-sectional view of suction electrode in place, showing penetration of probe through epidermis, (c)Helical electrode, that is attached to fetal skin by corkscrew-type action. 53 Electrodes for detecting fetal electrocardiogram during labor, by means of intracutaneous needles BME 301 Lecture Note 3 - Ali Işın 2013

54. (a)Wire-loop electrode, (b)platinum-sphere cortical- surface potential electrode, (c)Multielement depth electrode. 54 Implantable electrodes for detecting biopotentials BME 301 Lecture Note 3 - Ali Işın 2013

55. (a)One-dimensional plunge electrode array (after Mastrototaro et al., 1992), (b)Two-dimensional array, and (c)Three-dimensional array (after Campbell et al., 1991). 55 Examples of microfabricated electrode arrays BME 301 Lecture Note 3 - Ali Işın 2013

56. Capacitance per unit length  0 = dielectric constant of free space  r = relative dielectric constant of insulation material D = diameter of cylinder consisting of electrode plus insulation D = diameter of electrode L = length of shank 56 The structure of a metal microelectrode for intracellular recordings BME 301 Lecture Note 3 - Ali Işın 2013

57. (a)Metal-filled glass micropipet. (b)Glass micropipet or probe, coated with metal film. 57 Structures of two supported metal microelectrodes BME 301 Lecture Note 3 - Ali Işın 2013

58. (a)Section of fine-bore glass capillary, (b)Capillary narrowed through heating and stretching, (c)Final structure of glass-pipet microelectrode. 58 A glass micropipet electrode filled with an electrolytic solution BME 301 Lecture Note 3 - Ali Işın 2013

59. (a)Beam-lead multiple electrode. (b)Multielectrode silicon probe after Drake et al. (c)Multiple-chamber electrode after Prohaska et al. (d)Peripheral-nerve electrode based on the design of Edell. 59 Different types of microelectrodes fabricated using microelectronic technology BME 301 Lecture Note 3 - Ali Işın 2013

60. (a)Electrode with tip placed within a cell, showing origin of distributed capacitance, (b)Equivalent circuit for the situation in (a), (c)Simplified equivalent circuit. 60 Equivalent circuit of metal microelectrode BME 301 Lecture Note 3 - Ali Işın 2013

61. (a)Electrode with its tip placed within a cell, showing the origin of distributed capacitance, (b)Equivalent circuit for the situation in (a), (c)Simplified equivalent circuit. (From L. A. Geddes, Electrodes and the Measurement of Bioelectric Events, Wiley-Interscience, 1972. Used with permission of John Wiley and Sons, New York.) 61 Equivalent circuit of glass micropipet microelectrode BME 301 Lecture Note 3 - Ali Işın 2013

62. (a)Constant-current stimulation, (b)Constant-voltage stimulation. 62 Current and voltage waveforms seen with electrodes used for electric stimulation BME 301 Lecture Note 3 - Ali Işın 2013

63. 63 Simplified equivalent circuit of a Needle type EMG electrode pair and equivalent circuit of the input stage of an amplifier Needle type EMG electrode BME 301 Lecture Note 3 - Ali Işın 2013

64. Figure shows equivalent circuit of a biopotential electrode. A pair of these electrodes are tested in a beaker of physiological saline solution. The test consists of measuring the magnitude of the impedance between the electrodes as a function of frequency via low-level sinusoidal excitation so that the impedances are not affected by the current crossing the electrode–electrolyte interface. The impedance of the saline solution is small enough to be neglected. Sketch a Bode plot (log of impedance magnitude versus log of frequency) of the impedance between the electrodes over a frequency range of 1 to 100,000 Hz. 64 Example BME 301 Lecture Note 3 - Ali Işın 2013

65. Solution 65 Assume Figure in previous slide models both electrodes of the pair. The low corner frequency is Fc = 1/(2  RC) = 1/(2  ·20 k  ·100 nF) = 80 Hz. The high corner frequency is Fc = 1/(2  RC) = 1/(2  ·20 k  ||300  ·100 nF) = 5380 Hz. The slope between the two corner frequencies is –1 on a log-log plot. BME 301 Lecture Note 3 - Ali Işın 2013

66. A pair of biopotential electrodes are implanted in an animal to measure the electrocardiogram for a radiotelemetry system. One must know the equivalent circuit for these electrodes in order to design the optimal input circuit for the telemetry system. Measurements made on the pair of electrodes have shown that the polarization capacitance for the pair is 200 nF and that the half-cell potential for each electrode is 223 mV. The magnitude of the impedance between the two electrodes was measured via sinusoidal excitation at several different frequencies. The results of this measurement are given in the accompanying table. On the basis of all of this information, draw an equivalent circuit for the electrode pair. State what each component in your circuit represents physically, and give its value. 66 Example BME 301 Lecture Note 3 - Ali Işın 2013

67. Solution 67 The 600  is the tissue impedance plus the electrode/electrolyte high- frequency interface impedance. The 19400  is the electrode/electrolyte low-frequency interface impedance. The 200 nF is the electrode/electrolyte interface capacitance. The 223 mV is the electrode/electrolyte polarization voltage. BME 301 Lecture Note 3 - Ali Işın 2013