1. LPPD University of Illinois Chicago, LPPP. Summer 2008 Magnetically-Guided Nanoparticles for Targeted Drug Delivery Presentation for RET program July 7, 2011 Seth Baker Advisors: Dr. Andreas Linninger, Eric Lueshen, Madhawa Hettiarachchi Laboratory for Product and Process Design University of Illinois- Chicago Department of Chemical Engineering University of Illinois Chicago, LPPD, Summer 2011

2. LPPD University of Illinois Chicago, LPPP. Summer 2008 Project Summary Neurological therapeutics have limited success due to natural barriers such as the blood brain barrier. Methods to circumvent or penetrate the blood brain barrier include: Receptor- Mediated TransportMolecular Trojan Horses IntranasalOsmotic Disruption Convection-Enhanced DeliveryMacrophage Transport Over 7 million Americans suffer from neurological conditions such as Alzheimer’s, Parkinson’s, Brain Cancer, and Stroke. These conditions have direct and indirect costs of over $200 billion dollars annually in the United States. Magnetically – guided nanoparticles could offer targeted detection, diagnosis and treatment options for these neurological conditions. University of Illinois Chicago, LPPD, Summer 2011

3. LPPD University of Illinois Chicago, LPPP. Summer 2008 Benefits of Magnetic Nanoparticles Nanoparticles can be coated or loaded with various therapeutics. University of Illinois Chicago, LPPD, Summer 2011 Allows for more targeted drug delivery resulting in lower dosage and systemic toxicity. Particles are biocompatible and biodegradable.

4. LPPD University of Illinois Chicago, LPPP. Summer 2008 Determine Magnetic Quality University of Illinois Chicago, LPPD, Summer 2011 Time = 0 sec Time = 60 sec Time = 120 secTime = 180 sec Time = 240 sec Time = 300 sec Time = 360 sec Time = 420 sec Magnetite nanoparticles were guided toward a 173 lb pull force magnet over an 8 minute experiment.

5. LPPD University of Illinois Chicago, LPPP. Summer 2008 Agarose Gel Experimental Design University of Illinois Chicago, LPPD, Summer 2011 0.5 % Agarose gel 173 lb pull force magnet Syringe loaded with magnetite nanoparticles ? Control Petri dish

6. LPPD University of Illinois Chicago, LPPP. Summer 2008 Agarose Gel Early Results University of Illinois Chicago, LPPD, Summer 2011 Control 35 lb pull force 173 lb pull force Base of petri dish Syringe line Magnetic nanoparticles were guided toward the magnets through 0.5% agarose gel

7. LPPD University of Illinois Chicago, LPPP. Summer 2008 Rat Brain Experiments University of Illinois Chicago, LPPD, Summer 2011 Determining permeability of Prussian blue stain on unfixed rat brain Rat brain in saline solution Rat brain in Prussian blue stain Coronal cross section with no staining Set up for magnetic nanoparticle movement on unfixed rat brain Coronal cross section of rat brain Adding 10 µ l magnetite nanoparticles 173 lb pull force magnet and a control

8. LPPD University of Illinois Chicago, LPPP. Summer 2008 Future Work Continue with magnetic nanoparticle experiments to reduce agglomeration of nanoparticles and allow for convection enhanced delivery. Determine the most effective protocol for administrating and measuring magnetically-guided nanoparticles in rat brain samples. Investigate methods of manufacturing nanoparticles for better visualization in angiogram – possible gold plated magnetite. University of Illinois Chicago, LPPD, Summer 2011

9. LPPD University of Illinois Chicago, LPPP. Summer 2008 Acknowledgements NSF EEC-0502272 Grant, Chicago Science Teacher Research Dr. Andreas Linninger Members of the LPPD – Eric Lueshen, Sukhi Basati, Joe Kanikunnel University of Illinois Chicago, LPPD, Summer 2011