1. Date of download: 6/27/2016 Copyright © ASME. All rights reserved. From: A Simplified Procedure to Determine the Optimal Rate of Freezing Biological Systems J Biomech Eng. 2004;127(2):295-300. doi:10.1115/1.1865213 The general variation of the predicted optimal cooling rate (Bopt,°C∕min) as a function of reference membrane permeability (Lpg,μm∕min-atm; ranging from 0 to 1.0μm∕min-atm). Bopt values are plotted by assuming SA∕WV=1.0μm−1 and at two different values of ELp (10.0 and 20.0Kcal∕mole). The Lpg values are shown along the x-axis and the Bopt values are shown along the y-axis. Figure Legend:

2. Date of download: 6/27/2016 Copyright © ASME. All rights reserved. From: A Simplified Procedure to Determine the Optimal Rate of Freezing Biological Systems J Biomech Eng. 2004;127(2):295-300. doi:10.1115/1.1865213 Plot of the predicted optimal cooling rate (Bopt,°C∕min) as a function of water volume/surface area (SA∕WV,μm−1) at different ELp values and at Lpg=1.0μm∕min-atm. The SA∕WV values are shown along the x-axis and Bopt values are shown along the y-axis in logarithmic scale. Figure Legend:

3. Date of download: 6/27/2016 Copyright © ASME. All rights reserved. From: A Simplified Procedure to Determine the Optimal Rate of Freezing Biological Systems J Biomech Eng. 2004;127(2):295-300. doi:10.1115/1.1865213 Generic optimal cooling rate chart (GOCRC). Predicted GOCRC cooling rates are plotted as a function of activation energy (ELp,Kcal∕mole) at Lpg=1.0μm∕min-atm and SA∕WV=1μm−1. The dotted line represents a curve fitting used to generate a mathematical equation for BGOCRC value, BGOCRC=1009.5e−0.0546ELp (with a goodness of fit of R2=0.994). The ELp values are shown along x-axis and BGOCRC values are shown along y-axis in logarithmic scale. Figure Legend: