1. BIOCHEMICAL MARKERS PAL (PHENYLALANINE AMMONIA LYASE), G-POD (GUAIACOL PEROXIDASE), APX (ASCORBATE PEROXIDASE), PPO (POLYPHENOL OXIDASE). MOLECULAR MARKERS PR1, PR5 (PATHOGENESIS RELATED PROTEINS). RESISTANCE PATHOGEN OR ELICITOR EXPOSURE SYSTEMIC SIGNAL

2. WORKPLAN 1° PHASE – CHITOSAN AS AN ELICITOR OF SYSTEMIC ACQUIRED RESISTANCE. STUDY ON A MODEL SYSTEM: IN VITRO CULTURES. CHITOSAN – HOST PLANT INTERACTION 2° PHASE – CHITOSAN AS AN ELICITOR OF SYSTEMIC ACQUIRED RESISTANCE CHECKING THE TREATMENT’S EFFECTIVENESS ON 2YRS OLD PLANTS IN THE PRESENCE OF THE PATHOGEN PSEUDOMONAS SYRINGAE PV. ACTINIDIAE

3. MULTIPLICATION PHASE IN VITRO CULTURES BIOCHEMICAL MARKERS a b b a a a a b a ab b

4. a b b a b a b a b c ROOTING PHASE IN VITRO CULTURES BIOCHEMICAL MARKERS

5. IN VITRO CULTURES MOLECULAR MARKERS

6. IN VITRO CULTURES MOLECULAR MARKERS: PR1

7. IN VITRO CULTURES MOLECULAR MARKERS: PR5

8. 2° PHASE – CHITOSAN AS AN ELICITOR OF SYSTEMIC ACQUIRED RESISTANCE CHECKING THE TREATMENT’S EFFECTIVENESS ON 2 YRS OLD PLANTS IN THE PRESENCE OF THE PATHOGEN PSEUDOMONAS SYRINGAE PV. ACTINIDIAE Fratarcangeli et al., 2010 1° FASE – CHITOSANO COME ELICITORE DELLE DIFESE IMMUNITARIE DELLA PIANTA: STUDIO SU UN SISTEMA MODELLO INTERAZIONE CHITOSANO – KIWI (COLTURE IN VITRO) WORKPLAN

9. 2 YEARS OLD PLANTS BIOCHEMICAL MARKERS GPOD APX

10. PAL PPO 2 YEARS OLD PLANTS BIOCHEMICAL MARKERS

11. NT NI not treated, not inoculated; T NI treated, not inoculated; NT I not treated, inoculated; T I treated, inoculated. a ab b c 2 YEARS OLD PLANTS BIOCHEMICAL MARKERS

12. NT NI not treated, not inoculated; T NI treated, not inoculated; NT I not treated, inoculated; T I treated, inoculated.

13. TREATMENT ALONG THE NURSERY CHITOSAN MULTIPLICATION PHASE IN VITRO CULTURES ROOTING PHASE IN VITRO CULTURES TRANSPLANTATION AND GROWTH IN GREENHOUSE 2 YEARS OLD PLANT TRANSFER IN THE FIELD AND CHECKING THE EFFECTIVENESS OF THE TREATMENT IN THE PRESENCE OF NATURAL INOCULUM 1 2 3 4 5 CHITOSAN