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Presentation on theme: "/2011/07/02/say-cheese-for-scanning-electron-microscopy/ Life Science Mr."— Presentation transcript:

1 http://tysontrepidations.wordpress.com /2011/07/02/say-cheese-for-scanning-electron-microscopy/ http://www.ijs.si/~goran/semmate3.html Life Science Mr. Hooper September 2015

2 http://www.design-laorosa.com/2010/12/incredible-photos-taken-with-scanning.html Velcro

3 http://www.design-laorosa.com/2010/12/incredible-photos-taken-with-scanning.html Butterfly eggs

4 Influenza virus particles

5 Bread mold http://www.barrie-tao.com/microscope_photo.html penny

6 Salt and PepperPollen grains

7 http://www.topdesignmag.com/25-amazing-electron-microscope-images/ Microscopic Images Common House Fly Scanning Electron Microscope (SEM)

8 Most light microscopes have two glass lenses and are called compound microscopes. http://www.biologycorner.com/bio1/microscope.html The light microscope is an instrument that uses light to produce an enlarged image of the object being examined. Light microscopes can magnify an object up to 1000 times.

9 http://www.biologycorner.com/bio1/microscope.html Eyepiece: to look through. Magnifies 10x. Body tube: Maintains correct distance between eyepiece lens and objective lens. Coarse adjustment: Moves body tube or stage up and down; large movements. Fine adjustment: moves body tube or stage very small distance; fine focusing. Revolving Nosepiece: Holds objective lenses; revolves. Scanning objective: shortest objective (4x). Low power objective: short objective (10x). High power objective: long objective (40x).

10 http://www.biologycorner.com/bio1/microscope.html Arm: Supports body tube. Stage: platform that holds specimen slides. Stage clips: Holds slide to stage. Diaphragm: controls amount of light passing through specimen. Mirror/Light Source: illuminates specimen. Base: Supports microscope.

11 Eyepiece magnificationObjective magnificationTotal magnification 4.10x400x 5.100x500x 6.8x50x What is the total magnification when: 1.Eyepiece is 10x and objective is 10x? 2.Eyepiece is 10x and objective is 50x? 3.Eyepiece is 6x and objective is 100x? Complete this table (4-6)

12 History of the Microscope http://img.docstoccdn.com/thumb/orig/2192230.png

13 Anton van Leeuwenhoek Leeuwenhoek was a Dutch businessman who sold cloth, and in his spare time built simple microscopes. Leeuwenhoek is credited with discovery of bacteria, protozoa, algae, red blood cells, and spermatozoa. All of these he observed with his simple microscopes.

14 Anton van Leeuwenhoek Leeuwenhoek studied 67 kinds of insects as well as spiders. He examined life cycles from egg to larva to adult. Examined various types of tissues including muscle, bone, and nerve. First to observe RBCs, bacteria, protozoa, spermatozoa, nematodes, rotifers. October 24, 1632

15 http://www.digitalsmicroscope.com/scanning-electron-microscope-5/ Sends electrons over the surface of a specimen. Produces a three- dimensional image. SEMs can magnify a specimen up to 150,000 times.

16 http://images.fineartamerica.com/images-medium-large/daddy-long-legs-spider-head-sem-steve-gschmeissner.jpg Cellar spider

17 http://transmissionelectronmicroscope.org/ Send electrons through a very thinly sliced specimen. TEMs can magnify a specimen up to 500,000 times.

18 Bacillus subtilis - bacteriaplant cell

19 Does not harm living specimens, like electron microscopes. Uses sound waves to “see” an object. Can be used to examine living cells and for cancer detection.

20

21 Ballistic Forensics Investigators can examine the striations on bullets to determine if they were fired from a particular gun. CSI – Crime Scene Investigation Trace evidence such as hair and fibers can be examined microscopically.

22 leukemia African Sleeping Sickness parasites

23 http://www.biologycorner.com/worksheets/microbezoo_key.html Microscopic stream analysis of the number and diversity of living things in a particular area over time.

24 HIV (human immunodeficiency virus) particles, colored transmission electron micrograph (TEM). Each particle consists of an RNA (ribonucleic acid, purple) genome, surrounded by a protein nucleocapsid (coat, yellow). This is enclosed in a matrix of proteins (grey) and the whole virus is enclosed in an envelope (purple). http://www.sciencephoto.com/media/87184/enlarge

25 Preparing a Wet Mount Slide http://www.greatscopes.com/act005.gif http://geoclasses.tamu.edu/geography/bbednarz/tes/kdk/ See Appendix B Pg. 197 of Cells and Heredity

26 Anton van Leeuwenhoek. http://www.vanleeuwenhoek.com/. Bacillus subtilis.TEM. http://www.corbisimages.com/stock-photo/rights-managed/42-24897732/endospores-in- bacillus-subtilis-bacteria-tem.http://www.corbisimages.com/stock-photo/rights-managed/42-24897732/endospores-in- bacillus-subtilis-bacteria-tem Balloons. http://www.justusfourblog.com/wp-content/uploads/2013/01/Balloons.jpg. Bullet with striations. http://sassy3421.blogspot.com/2011/05/caught-by-bullet.html. Chronic Lymphycytic leukemia. http://pathwiki.pbworks.com/w/page/14674003/HemaSlide23. Common House Fly. http://www.topdesignmag.com/25-amazing-electron-microscope-images/http://www.topdesignmag.com/25-amazing-electron-microscope-images/ Hair. http://shs.westport.k12.ct.us/forensics/09-trace_evidence/splitting_hairs.htm. Healthy Blood Smear. http://www.sciencephoto.com/media/304706/enlarge.http://www.sciencephoto.com/media/304706/enlarge Influenza virus http://www.sciencebuzz.org/blog/viva-la-evolution-antibodies-show-darwins-theory-work. Leeuwenhoek microscope. http://biolulia.files.wordpress.com/2011/09/leeuwenhoek_microscope.png.http://biolulia.files.wordpress.com/2011/09/leeuwenhoek_microscope.png Leeuwenhoek portrait. http://www.vanleeuwenhoek.com/images/Antonie%20van%20Leeuwenhoek- Jan%20Verkolje-1632-1675.jpg.http://www.vanleeuwenhoek.com/images/Antonie%20van%20Leeuwenhoek- Jan%20Verkolje-1632-1675.jpg Rotifer. http://www.fcps.edu/islandcreekes/ecology/rotifer.htm. Penicillium sp. http://www.inspectapedia.com/sickhouse/MicroscopicMold.htm.http://www.inspectapedia.com/sickhouse/MicroscopicMold.htm Pollen. http://1.bp.blogspot.com/- 3cwHBJMhY3A/UWYwIYFaVZI/AAAAAAAAH7c/k9fh78P13I8/s1600/pollen.jpg Salt and Pepper SEM. http://www.topdesignmag.com/25-amazing-electron-microscope-images/http://www.topdesignmag.com/25-amazing-electron-microscope-images/ SAM of bone. http://bcrt.charite.de/index.php?id=825.http://bcrt.charite.de/index.php?id=825 SAM Set Up. http://www.hellotrade.com/acoustical-technologies-singapore-pte-singapore/scanninghttp://www.hellotrade.com/acoustical-technologies-singapore-pte-singapore/scanning -acoustic-microscope.html T.Brucei blood smear. http://www.healthhype.com/sleeping-sickness-african-trypanosomiasis.html.http://www.healthhype.com/sleeping-sickness-african-trypanosomiasis.html TEM. Plant cell. http://www.cas.miamioh.edu/~meicenrd/anatomy/ch2_ultrastructure/Tempcell.htm.


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