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AdvanSure ® for diagnosis of specific IgE Park, Jung-Won Allergy-Asthma Clinic, Severance Hospital.

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Presentation on theme: "AdvanSure ® for diagnosis of specific IgE Park, Jung-Won Allergy-Asthma Clinic, Severance Hospital."— Presentation transcript:

1 AdvanSure ® for diagnosis of specific IgE Park, Jung-Won Allergy-Asthma Clinic, Severance Hospital

2 Food Allergy  Anaphylaxis type  Atopic dermatitis  Eosinophil associated gastrointestinal disorder Heterogeneity of Th2 cell: IL-5 + (IL-4 +, IL-5 + ) vs. IL-5 - (IL-4 +, IL-5 - )

3 Allergic eosinophilic gastroenteritis is associated with greater frequency of peanut specific IL-5 producing T cells Prussin C et al J Allergy Clin Immunol 2009

4 Mast Cell Bioactive mediators of three categories

5

6 Schematic of original Radioallergosorbent Test

7

8  History  Resolution of skin symptoms with complete elimination  Development of symptoms following challenge  Skin test, sIgE test  DBPCFC Diagnosis of Food Allergy

9 Food Specific Ige (immuno-CAP RAST) values at /or above which there is a 95% risk of Clinical Allergy (no challenge necessary) Food Serum IgE (kIU/L) for 95% PPV Egg (child) >7 Cow’s milk (child) >15 Peanut >14 Fish >20

10 중요한 음식물 알레르겐 Classical allergens 땅콩 콩 달걀 우유 밀가루 : gliadine 견과류 : 호두, 아몬드 갑각류 : 새우, Lobster 생선 : 대구 메밀 Non-classical allergens (Oral allergy syndrome) 복숭아 사과 키위 생밤 참외

11 Allergen Panel of AdvanSure

12 Allergen Sources

13 Allergen Purification and Modification 기술

14 Allergen Quality Control

15 Production of Allergen-Coated Strips

16  Chip reader 개발  Data processing program 개발

17 AdvanSure sIgE 진단 kit  Detection limit: 0.34 KU/L  Result range: Negative (<0.35 KU/L) ~ <100 KU/L  Serum requirement: 100 μL  Processing time: 3 hr

18 Analysis of discrepant results btw AdvanSure and RIDA compared with ImmunoCAP (n=633) Agreement rate btw AdvanSure and RIDA: 73.9% Oh EJ, et al. 2010 Kor J Lab Med

19 AdvanSure® system showed good detection performance compared with ImmunoCAP® system in correlation and agreement. Upgrading of allergen item in panel may be needed. Conclusion


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