1. The Compound Light Microscope

2. What is a compound light microscope?
Mechanism for enlarging
an image

3. Evolution of the microscope
Choice of lens
magnification
2 set lenses

4. The Parts of the Microscope and Their Function
Maintains
proper distance
between lenses
Magnification
Rotates the objectives
Support body tube
Magnification
Supports slide
Holds slide
In place
Focuses image
Regulates
amount of light
Sharpens the image
Reflects light
towards eyepiece
Supports microscope

5. Parts of a Microscope Rotates the objectives
Objectives, magnifies images
a. Scanning (4X)
b. Low Power (10X)
c. High Power (40X)
3. Regulates the amount of light

6. Parts of a Microscope Lens that you look through (10X)
Focuses the image
Sharpens the image (only on high power)

7. Magnification The two lenses are called:
Eyepiece
Objective
The eyepiece has a magnification of “10x”
The magnification of the objective lenses vary and are marked on the lens
Total magnification =
(Eyepiece) X (Objective)

8. 10X
x =
40X
400X

9. 10X 4X 40X 10X 10X 100X 10X 40X 400X Eyepiece Power Objective Power
Total Magnification
Scanning
Low Power
High Power
10X 4X
40X
10X
10X
100X
10X
40X
400X

10. How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Lower the cover slip on top of the drop to prevent air bubbles
Cover Slip
Lower slowly

11. Let’s Review! Eyepiece Body tube Nosepiece Arm Scanning Low Power9.
10.
11.
14.
Eyepiece 1. 2. 3. 4. 5. 6. 7. 8.
Body tube
Nosepiece
Arm
Scanning
Low Power
Stage
High power
Stage clips
12.
Course adjustment
Diaphragm
13.
Fine adjustment
Light source
Base

12. So Let’s Review Let’s Review! Eyepiece Body tube Nosepiece Arm
Scanning
Low Power
Stage
High power
Stage clips
Course adjustment
Diaphragm
Fine adjustment
Light source
Base

13. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

14. Pg. 83