1. AP Biology

2. Nucleic Acids  Function:  store & transmit hereditary information  Examples:  RNA (ribonucleic acid)  DNA (deoxyribonucleic acid)  Structure:  monomers = nucleotides

3. 2005-2006 AP Biology Nucleotides  3 parts  nitrogen base (C-N ring)  pentose sugar (5C)  ribose in RNA  deoxyribose in DNA  PO 4 group

4. 2005-2006 AP Biology Types of nucleotides  2 types of nucleotides  different Nitrogen bases  purines  double ring N base  adenine (A)  guanine (G)  pyrimidines  single ring N base  cytosine (C)  thymine (T)  uracil (U)

5. 2005-2006 AP Biology Building the polymer

6. 2005-2006 AP Biology Nucleic polymer  Backbone  sugar to PO 4 bond  phosphodiester bond  new base added to sugar of previous base  polymer grows in one direction  N bases hang off the sugar-phosphate backbone Why is this important?

7. AP Biology RNA & DNA  RNA  single nucleotide chain  DNA  double nucleotide chain  N bases bond in pairs across chains  spiraled in a double helix  double helix 1 st proposed as structure of DNA in 1953 by James Watson & Francis Crick (just celebrated 60th anniversary)

8. 2005-2006 AP Biology Pairing of nucleotides  Nucleotides bond between DNA strands  H bonds  purine :: pyrimidine  A :: T  2 H bonds  G :: C  3 H bonds Why is this important?

9. 2005-2006 AP Biology Information polymer  Function  series of bases encodes information  like the letters of a book  stored information is passed from parent to offspring  need to copy accurately  stored information = genes  genetic information

10. 2005-2006 AP Biology

11. DNA molecule  Double helix  H bonds between bases join the 2 strands  A :: T  C :: G Why is it important that the strands are bonded by H bonds?

12. AP Biology Copying DNA  Replication  2 strands of DNA helix are complementary  have one, can build other  have one, can rebuild the whole  why is this a good system?  when in the life of a cell does replication occur?  mitosis  meiosis

13. 2005-2006 AP Biology DNA replication “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” James Watson Francis Crick 1953

14. 2005-2006 AP Biology Watson and Crick … and others… 1953 | 1962

15. 2005-2006 AP Biology Maurice Wilkins… and… 1953 | 1962

16. AP Biology Rosalind Franklin Rosalind Franklin (1920-1958)

17. AP Biology Interesting note…  Ratio of A-T::G-C affects stability of DNA molecule  2 H bonds vs. 3 H bonds  biotech procedures  more G-C = need higher T° to separate strands  high T° organisms  many G-C  parasites  many A-T (don’t know why)

18. AP Biology Macromolecule Review

19. AP Biology Carbohydrates  Structure / monomer  monosaccharide  Function  energy  raw materials  energy storage  structural compounds  Examples  glucose, starch, cellulose, glycogen glycosidic bond

20. AP Biology Lipids  Structure / building block  glycerol, fatty acid, cholesterol, H-C chains  Function  energy storage  membranes  hormones  Examples  fat, phospholipids, steroids ester bond (in a fat)

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22. AP Biology Proteins  Structure / monomer  amino acids  levels of structure  Function  enzymes ◆ defense  transport ◆ structure  signals ◆ receptors  Examples  digestive enzymes, membrane channels, insulin hormone, actin peptide bond

23. Know the Differences Between Levels of Protein Structure!

24. Denaturation of Proteins 24

25. Changing Tertiary Structure… 25

26. Denaturation and the Active Site 26

27. AP Biology Nucleic acids  Structure / monomer  nucleotide  Function  information storage & transfer  Examples  DNA, RNA phosphodiester bond

28. Why does ethanol precipitate DNA?  DNA is negatively charged because of phosphate backbone  Ion-dipole interactions between water and phosphate stabilize DNA structure  Lots of ethanol removes water of hydration around phosphates  Na + ions (from salt) step in and force changes in DNA structure, leading to precipitation  Wiki article on ethanol precipitation very helpfulethanol precipitation 28

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30. Denatured (precipitated) DNA 30

31. Grid In Question on Nucleic Acids  A scientist extracts DNA from the nucleus of cells and sequences it. The scientist determines that 27% of the nucleotide bases are guanine. What percentage of the bases are thymine?  In DNA, G=C and A=T  If 27% are G, then 27% are C: 54% G=C  Therefore 46% of bases are A=T;  23% thymine and 23% adenine 31

32. Practice Grid-in 2  A sample of DNA contains 21% adenine. What percentage of the DNA is cytosine? 32