1. ArabLab 2012 Dubai 26.-29. Apr. 2012 FTIR and NDIR Spectroscopy Measurements on ambient air and human breath F. Jäger, O. Gluschke, R. Doll Delta Analytics OHG Fahrenheitstr. 1 28359 Bremen, Germany www.delta-analytics.de

2. Content in brief: The company Infrared Spectroscopy Applications Results Visit us @ booth 430 ArabLab 2012 Dubai 26.-29. Apr. 2012

3. The Company Scope: high finesse instruments for measurements of isotope ratios und trace gases Technology compound BITZ close to University of Bremen GERMANY ArabLab 2012 Dubai 26.-29. Apr. 2012

4. Method: Infrared Spectroscopy  Attenuation of a broad band light source inside the gas sample  Detects only molecules with a net change of the dipole moment (no linear molecules like O 2, N 2, He) 1. None-Dispersive-Infra-Red (NDIR) technique to measure δ 13 C, CO 2 2. Fourier-Transform-Infra-Red (FTIR) technique to measure δ 13 C, CO 2, CH 4, N 2 O, CO,... ArabLab 2012 Dubai 26.-29. Apr. 2012

5. Applications 1. Environment  Environmental air, to monitor climate change  Respiration of plants, to study their life cycle and monitor influence of the surrounding  Interaction of oceans, vegitation and soil with the atmosphere  Vulcanic activity 2. Breath Analysis  Examine metabolism / organ functions in the body of humans and animals ArabLab 2012 Dubai 26.-29. Apr. 2012

6. Hitran spectra of CO 2 2250 cm -1 - 2400 cm -1 P-branchR-branch 13 CO 2 12 CO 2 ArabLab 2012 Dubai 26.-29. Apr. 2012

7. 1. DIA Delta Isotope Analyser NDIR non dispersive infrared = opto acoustic technique with ABB Uras26 analyser Temperature stabilized two fold. Inner core optical unit + outer box. Humidity filterd two fold. Nafion tube + perchlorate Easy to use software, platform independent, web application optinon Power supply by wind or solar energy Rugged design

8. ArabLab 2012 Dubai 26.-29. Apr. 2012 Gas Flow NDIR DIA

9. ArabLab 2012 Dubai 26.-29. Apr. 2012 2. DITA Delta Isotope and Trace Gas Analyser FTIR Fourier transform infrared optical technique = Bruker spectrometer 'alpha', the world's smallest FTIR Temperature stabilized two fold. Inner core optical unit + outer box. Humidity filterd two fold. Nafion tube + perchlorate Easy to use software, platform independent, web application optinon Power supply by wind or solar energy

10. Measurement sequence on the DIA (NDIR) instrument 3 reference gases + outside air (measurements at the Univ. Groningen) Time [sec] C1 = 405 ppm,  =-9.44 C 2 = 458 ppm,  =-11.62 C 3 = 309 ppm,  =-7.84 Results ArabLab 2012 Dubai 26.-29. Apr. 2012 Results NDIR DIA

11. Allan deviation plot Delta Analytics „DIA“   A (k  2m) -1  [A s+1 (k) - A s (k) ] 2 (Werle et al, 1993) k= number of measurements, s=1...m (subset) Results ArabLab 2012 Dubai 26.-29. Apr. 2012 Results NDIR DIA

12. CO N2O 13CO2 CH4 FTIR spectrum on synthetic air Delta Analytics „DITA“ Res. 2 cm -1 15 scans (5 sec) 12CO2 Results ArabLab 2012 Dubai 26.-29. Apr. 2012 Results FTIR DITA

13. Analysis of FTIR spectra – MALT/CLS (D. Griffith) software – mathematical treatment of theoretical and measured spectra Any calculation will have to incorporate – Multivariate analysis. SVD or PCA – Simulation of theoretical spectra (HITRAN) – Fitting of spectra to a theoretical curve – Calibrating Measured Results with Calibration Data ArabLab 2012 Dubai 26.-29. Apr. 2012

14. Comparison of the precision of NDIR and FTIR Environmental analysers NDIR D.A. FTIR D.A. 5m FTIR Griffith 20m FTIR EchoT. 24m Mixing ratio CO2 [μmol/mol]0.050.50.150.05 CH4 [nmol/mol]-< 50.90.2 N2O [nmol/mol]-< 50.3< 0.06 CO [nmol/mol]-< 50.30.2 δ 13 C per mil [‰] 0.2< 0.50.1< 0.08 D.A. and Griffith 2000: 2 minutes sampling, 1  standard deviation EcoTech.: 10 minutes sampling, 1  standard deviation ArabLab 2012 Dubai 26.-29. Apr. 2012

15. Breath Test 1. Take Basal Breath Sample 2. Eat Test Meal, including 13 C Substrate 3. Take further Breath Samples at defined Time intervals (i.e. 30 min, 60 min, 90, min,...) 4. Do Measurements on DIBTA 5. Print Test Report

16. DIBTA Delta Isotope Breath Test Analyser Analysis of human breath δ 13 C precision : 0.1 ‰ Helicobacter Pylori Liver function, Pankreas function Digestion (corn starch, fructose) Gastric emptying Energy expenditure Lactose deficiency ArabLab 2012 Dubai 26.-29. Apr. 2012

17. Thank you to the cooperation partners – Prof. H.A.J Meijer (University of Groningen) – Prof. Erik Kerstel (University of Grenoble) – Prof. Willi Brand (MPI BGC in Jena) – Dr. Michael Paul Thank you for your attention :-) ArabLab 2012 Dubai 26.-29. Apr. 2012