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Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects.

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Presentation on theme: "Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects."— Presentation transcript:

1 Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects Arch Ophthalmol. 2003;121(4):547-557. doi:10.1001/archopht.121.4.547 Schematic diagram of intraretinal oxygen measurements. A double-barreled microelectrode is used as described elsewhere by Linsenmeier and Yancey and inserted into the vitreous through a 15-gauge needle mounted onto a manipulator that allows gonioscopic motion. A boot system is installed to maintain intraocular pressure and to prevent vitreal leakage. The microelectrode is then connected to a microdrive that controls movement in and out of the retina. One barrel, filled with isotonic sodium chloride solution and connected to an amplifier via a silver/silver chloride (Ag/AgCl) wire, measures voltages generated in the retina (the intraretinal electroretinogram and the transepithelial potential across the retinal pigment epithelium). The copper wire from the other barrel is connected to a polarizing voltage (−0.7 V) and a picoammeter to measure currents proportional to PO 2. Figure Legend:

2 Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects Arch Ophthalmol. 2003;121(4):547-557. doi:10.1001/archopht.121.4.547 Intraretinal oxygen profiles across cat retina during light and dark adaptations. The retina is shown schematically at the top. The 4 cell types shown are (from left to right) retinal pigment epithelial cells, rod photoreceptors, bipolar cells, and ganglion cells. Figure Legend:

3 Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects Arch Ophthalmol. 2003;121(4):547-557. doi:10.1001/archopht.121.4.547 Frequency histogram of the distribution of inner retinal PO 2 in dark adaptation. The mean ± SD are 18.42 ± 12.52. Reproduced from The Journal of General Physiology (1992;99:177-197), by copyright permission from The Rockefeller University Press. Figure Legend:

4 Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects Arch Ophthalmol. 2003;121(4):547-557. doi:10.1001/archopht.121.4.547 Intraretinal oxygen profiles recorded in dark during normoxia and hypoxemia. Reproduced from The Journal of General Physiology (1992;99:177-197), by copyright permission from The Rockefeller University Press. Figure Legend:

5 Date of download: 5/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Retinal Oxygen: Fundamental and Clinical Aspects Arch Ophthalmol. 2003;121(4):547-557. doi:10.1001/archopht.121.4.547 Intraretinal oxygen profiles in dark during normoxia and hyperoxia. Figure Legend:


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