1. PowerPoint Slides for Chapter 1: Heritable Material by A. Malcolm Campbell, Laurie J. Heyer, & Christopher Paradise 1.5 Is all genetic information encoded linearly in the DNA sequence? Integrating Concepts in Biology Title Page Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

2. Biology Learning Objectives Describe the epigenetic code using methylcytosine and its effects on gene activity. Evaluate experimental design and analyze data from research on DNA as molecular information. Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

3. Normal Bases Fig. 1.18 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

4. Methylated Bases Fig. 1.18 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

5. Methylated Bases Fig. 1.18 different chemical structures different physical properties Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

6. Methylated Bases Fig. 1.18 these are NOT mutations! methylation is epigenetic change Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

7. Thin Layer Chromatography Fig. 1.19 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

8. Thin Layer Chromatography Fig. 1.19 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

9. Thin Layer Chromatography Fig. 1.19 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

10. Thin Layer Chromatography Fig. 1.19 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

11. Thin Layer Chromatography Fig. 1.19 Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.

12. Bases of Active vs Inactive DNA Fig. 1.20 from Naveh-Many and Cedar. 1981.

13. Bases of Active vs Inactive DNA Fig. 1.20 from Naveh-Many and Cedar. 1981.

14. Bases of Active vs Inactive DNA Fig. 1.20 What is the general rule about gene activity and methylation? from Naveh-Many and Cedar. 1981.

15. Bases of Active vs Inactive DNA Fig. 1.20 What is the general rule about gene activity and methylation? active genes are hypomethylated inactive genes are hypermethylated from Naveh-Many and Cedar. 1981.

16. Fig. 1.20 active genes are hypomethylated inactive genes are hypermethylated Cause vs Correlation from Naveh-Many and Cedar. 1981.

17. Pharmacological Gene Regulation Fig. 1.21 adult monkey responsed to methylase inhibitor Can genes be regulated by epigenetic changes? modified from DeSimone, et al., 1982

18. Pharmacological Gene Regulation Fig. 1.21 adult monkey responsed to methylase inhibitor fetal hemoglobin levels over time modified from DeSimone, et al., 1982

19. Pharmacological Gene Regulation Fig. 1.21 fetal hemoglobin in response to methylation inhibitor modified from DeSimone, et al., 1982

20. Pharmacological Gene Regulation Fig. 1.21 fetal hemoglobin in response to methylation inhibitor modified from DeSimone, et al., 1982

21. Pharmacological Gene Regulation Fig. 1.21 third (higher) dose of methylation inhibitor modified from DeSimone, et al., 1982

22. Pharmacological Gene Regulation Fig. 1.21 What is the consequence of higher inhibitor dose? modified from DeSimone, et al., 1982

23. Pharmacological Gene Regulation Fig. 1.21 What is the consequence of higher inhibitor dose? modified from DeSimone, et al., 1982

24. Pharmacological Gene Regulation Fig. 1.21 What is the consequence of higher inhibitor dose? modified from DeSimone, et al., 1982

25. Pharmacological Gene Regulation Fig. 1.21 Why was this a bad idea for clinical use? modified from DeSimone, et al., 1982

26. Fig. 1.21 Does this experiment show causation? Cause vs Correlation modified from DeSimone, et al., 1982

27. End of Chapter 1 Exam questions drawn from: IQs Review Questions End of Chapter Look at old tests. Use this weekend to catch up. Pace will pick up from now on. First exam in 2 weeks. Copyright © 2015 by AM Campbell, LJ Heyer, CJ Paradise. All rights reserved.