1. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR 3V Silk Road To Water Resistance

2. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com PRESENTATION ISSUES: Some General Properties Application in Cosmetics Application in Skin Care Emulsions: Sun Care Self-Tanning AHA Based Emulsions “In Vitro” Water Resistance Data Sensorial Data SYNTHALEN CR: The silk Road To WR

3. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com CHEMICAL AND PHYSICAL CHARACTERISTICS Appearancewhite to beige powder pH (1% water dispersion)3.5-5.5 Bulk density0.4-0.5 g/cm3 Viscosity of water dispersions INCI Name:Polyquaternium-37 %Viscosity Spindle 1.08000-16000 5 (Brookfield RVT, 20 rpm, 25°C) SYNTHALEN CR – General properties

4. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR Toxicological Profile Acute tox (oral, rat):LD50 > 2000 mg/kg Acute skin irritation (rabbit):non irritant Acute eye irritation (rabbit):non irritant Skin sensitization (maximization test): non sensitizing Ames Test: non mutagenic (with and without metabolic activation)

5. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Cationic emulsions are well known in the art thanks to their unique skin feel that is less tacky and more powdery than that of non- ionic and anionic ones. They can also help to improve the water resistance of the finished cosmetics, very important parameter in the case of emulsions for baby and sun care. Synthalen CR works very better in these systems and the data reported in this presentation will show that it is a versatile and cost-efficient alternative to other possible thickeners compatible with cationic environments. SYNTHALEN CR – Skin Care Emulsions

6. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com High SPF formulations Water Resistance Photostable UV Filters UV-A Protection Oil free Quick Absorption / Drying and lack of Greasiness Sun Care Trends in the European Market

7. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com WR Evaluation Outstanding Performance at low concentration

8. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR Cosmetic emulsion once applied onto the skin has a certain tendency to be re-emulsified when comes in contact with water. This tendency depends on: Kind of emulsion (O/W, W/O) Nature of emulsifier Amount of emulsifier Oil / Water Ratio Addition of specific ingredients

9. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Water Resistance: A key factor in sun protection in order to avoid re- emulsification of sun filters in contact with water, sweat, and so to assure a constant protection against UV Radiation. SYNTHALEN CR: The silk Road To WR

10. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR How to Evaluate Water Resistance: water resistant: SPF after 40 minutes of activity in water is more than 50% of initial SPF very water resistant: SPF after 80 minutes of activity in water is more than 50% of initial SPF EU: In Vivo test according to Colipa Guideline (2005) US: In Vivo test according to FDA Method For Product claimed WR or VWR, the SPF on the label is the SPF determined after 40 or 80 minutes of activity in water

11. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Use Of W/O emulsion Use of high Oil / Water Ratio Use of low level of hydrophilic emulsifiers (e.g: anionic, ethoxylated, etc) Use of silicones or high molecular weight emollients Use of hydrophobic film-forming polymers How to formulate Water Resistant Emulsion: SYNTHALEN CR: The silk Road To WR

12. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WRc Mechanism Water Resistant Additives:

13. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR Disadvantages of Old Fashioned WR Additives: Last Generation of WR actives work by creating a hydrophobic film of a high MW oil soluble polymer (usually a polyolefin) on the skin surface this. This kind of mechanism has some disadvantages: Negative effect on sensoriality of formulations Difficulties in formulating Occlusive effect on the skin Requires Hot Process

14. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR SYNTHALEN CR is an innovative solution for improving substantivity and water resistance of skin care formulation without using lipophilic ingredients: This hydrophilic polymer, thanks to its high MW and high cationic charge density, is able to create a not occlusive film that prevents the active ingredients (eg UV filters) washing off from the skin surface. The cationic nature of SYNTHALEN CR is the key for understanding its excellent substantivity to the skin and its unique sensorial profile. Application Patented by 3V SIGMA

15. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR In this test method, 100 mg of a sunscreen is applied on a glass sheet (50 cm 2 ) covered with a 3M Transpore Surgical Tape 1527 using a gloved finger. After 20’ glass sheet is immersed in water at 25 ± 1°C circulating to stimulate moderate activity. After 20’ of immersion the glass sheet is allowed to dry for 20’. The immersion/drying cycle is repeated to a total immersion time of 40’ (two immersions) and 80’ (four immersions). The same quantity is applied to another glass sheet as control. The composition is, then, removed from the tapes with ethanol. The Uvasorb HEB content of the two alcoholic solutions is measured by UV spectroscopy. The ratio of the absorbance values of the immersed and non-immersed glass sheets gives the UV filter retention. An average percentage retention is calculated from the results of 4 trials. In Vitro WR Test

16. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com I PEG-100 Stearate 0.40 Glyceryl Stearate 0.50 Dimethicone 1.00 Stearyl Alcohol 3.00 C 12-15 Alkyl Benzoate 2.00 Uvasorb HEB 3.00 BHT 0.10 Benchmark A - II H 2 O up to 100 SYNTHALEN CR 0.30 Carbomer - 3V Allantoin (Allantoin) 0.20 Glycerin 3.00 III AMP qs Phenoxyethanol and Parabens 1.00 Parfum 0.20 0.40 0.50 1.00 3.00 2.00 3.00 0.10 - up to 100 - 0.20 3.00 qs 1.00 0.20 0.40 0.50 1.00 3.00 2.00 3.00 0.10 1.00 up to 100 - 0.20 3.00 qs 1.00 0.20 SYNTHALEN CR: The silk Road To WR In Vitro WR Test

17. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR Synthalen CR is able to achieve Outstanding WR at only 0,3%! In Vitro WR Test

18. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR I. Distearyl Dimonium Chloride 3.00 Glyceryl Stearate 1.00 BHT0.10 Cetyl Alcohol 1.00 C12-15 Alkyl Benzoate 4.00 Ethylhexyl Palmitate 4.00 Propylene Glycol Dicaprylate Dicaprate 2.00 Uvasorb HEB 3.00 I.H2Oup to 100 SYNTHALEN CR0.30 3V Allantoin (Allantoin)0.20 Glycerin 3.00 III.Phenoxyethanol & Parabens 1.20 In Vitro WR Test Viscosity (Brookfield RVT, 20 rpm, 25°C):26000 mPa·s pH:5.5-6

19. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR In Vitro WR Test Non Ionic EM Cationic EM

20. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Sensory Evaluation Because Water Resistance not always means Tackiness

21. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Others littlemuch Cushion little much Distribution difficult easy Absorption slow fast Oiliness much little Waxiness much little Tackiness much little Smoothness little much Softness little much Formulation with 0,3% Synthalen CR vs Formulation with Benchmark A (0-line) -1 0 +1 SYNTHALEN CR: The silk Road To WR Sensory Evaluation 10 Volunteers, 23°C, 45% relative humidity.

22. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR: The silk Road To WR Sensory Evaluation Synthalen CR thanks to its cationic conditioning nature has the ability of improving the skin feel of formulations by: Reducing oiliness and tackiness sensation Improving Cushion during application An outstanding dry and silky after feel The sensorial propriety can be easily perceived in simple gel of polymer in water

23. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Stabilizing Effect A wide compatibility with most common chemical and mineral UV-filter and a unique stabilizing effect

24. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com THICKENING PROPERTIES Viscosity vs. concentration Brookfield RVT, 20 rpm, 25°C 100 1000 10000 100000 0.00.51.01.5 % of polymer Viscosity (mPas)

25. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Viscosity vs. pH 1% water dispersion THICKENING PROPERTIES 1000 10000 100000 23456789101112 pH Viscosity (mPas) Brookfield RVT, 20 rpm, 25°C

26. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SUSPENDING ABILITY Viscosity @ 0.5 rpm – Viscosity @ 1.0 rpm Yield Value (mPa): 100 Brookfield RVT, 25°C Viscosity of dispersions: ~10000 mPa·s 0 200 400 600 800 1000 Synthalen K Synthalen L Synthalen M PNC 400PNC 430 Stabylen 30 Synthalen W2000 Synthalen CR Yield Value (mPa)

27. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com EFFECT OF TEMPERATURE Viscosity vs. temperature 1% water dispersion 1000 10000 100000 2030405060 Temperature (°C) Viscosity (mPa?s)

28. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com SYNTHALEN CR – Application in Cosmetics Hair Fixative and/or Conditioning Gels Clear Rinse-Off and Leave-On Conditioners Rinse-Off and Leave-On Conditioning 0/W Emulsions Ethanol Based Products O/W Emulsions for Wet Wipes Gels and O/W Emulsions for Sun Care Gels and O/W Emulsions for Skin Care Self-Tanning Gels and O/W Emulsions

29. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Compatibility with micronised ZnO; Compatibility with TiO2; O/W emulsions with cationic emulsifiers; O/W emulsions with non-ionic emulsifiers; SYNTHALEN CR – Skin Care Emulsions Applications in Sun Care O/W Emulsions

30. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Applications in Sun Care O/W Emulsions

31. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com Applications in Sun Care O/W Emulsions

32. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com * Calculated SPF

33. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com The pH range of application of SYNTHALEN CR is very large: from 2 to 9, then it finds its natural application in self tanning products containing DHA, where a low pH is necessary to assure to the finished cosmetics a good shelf life SYNTHALEN CR – Skin Care Emulsions Applications in Self-Tanners

34. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com * Calculated SPF

35. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com AHAs are a group of natural and physiological substances, which can modulate skin keratinization and increase biosynthesis of dermal components. They find application in moisturizers, cleansers, sunscreens, anti-wrinkle creams, foundation creams. They work best at a pH between 3 and 4 (3.5 is the minimum pH recommended by the FDA, while the European SCCP recommends a minimum pH of 3.8 in the case of glycolic acid). SYNTHALEN CR works very well in AHAs based emulsions. Emulsions with a large range of viscosity value (from fluid to medium/high viscosity) can be prepared, characterised by very high storage stability, excellent water resistance and sensorial characteristics. SYNTHALEN CR – Skin Care Emulsions Applications in AHA-Based O/W Emulsions

36. 3V Sigma S.P.A. Via Torquato Tasso 58 24121 Bergamo Italy Tel +390354165255 Fax +390352395693 www.3vsigma.com