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Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus David Pajuelo, Chung-Te Lee, Francisco J. Roig, Manuel.

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Presentation on theme: "Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus David Pajuelo, Chung-Te Lee, Francisco J. Roig, Manuel."— Presentation transcript:

1 Host-Nonspecific Iron Acquisition Systems and Virulence in the Zoonotic Serovar of Vibrio vulnificus David Pajuelo, Chung-Te Lee, Francisco J. Roig, Manuel L. Lemos, Lien-I Hor, Carmen Amaro Infection and Immunity 2014, vol. 82(2) p731-744.

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12 (Ferroportin)

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14 Hydroxamate catechols pyoverdins

15 Ferric enterobactin (catechols) of E. coli

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18 Reporter Gene FUR operator FUR Reporter Gene Transcript RNA Polymerase High-copy plasmid with FUR operator FURTA: FUR Titration Assay.

19 Chrome Azurol S

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22 CAS indicator displacement assay for heavy metals. w/o siderophores + siderophores

23 Pajuelo D. et al. (2014) Table 3(b)

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28 Pajuelo D. et al. (2014) Fig. 1a

29 Pajuelo D. et al. (2014) Fig. 2

30 Pajuelo D. et al. (2014) Table 3(b)

31 Pajuelo D. et al. (2014) Table 3(d)

32 Pajuelo D. et al. (2014) Fig. 3

33 Pajuelo D. et al. (2014) Table 3(a)

34 Pajuelo D. et al. (2014) Fig. 4a

35 Pajuelo D. et al. (2014) Fig. 4b

36 Pajuelo D. et al. (2014) Table 3(c)

37 Pajuelo D. et al. (2014) Fig. 5 Fresh eel plasma + 200 uM FeCl3 HI-eel plasma Eel blood Human plasma

38 Pajuelo D. et al. (2014) Fig. 1b hupAvuuA

39 Pajuelo D. et al. (2014) Fig. 6a

40 Pajuelo D. et al. (2014) Fig. 6b

41 Pajuelo D. et al. (2014) Fig. 6c

42 Pajuelo D. et al. (2014) Table 4.

43 Pajuelo D. et al. (2014) Table 5.

44 Pajuelo D. et al. (2014) Table 6.

45 Pajuelo D. et al. (2014) Table 7.


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