1. pGLO Bacterial Genetics

2. E. Coli structure

10. Plasmid Additional DNA found in some bacteria Small loop Carries one or more genes Can be transferred from one bacteria to another.

11. pGLO plasmid (original form) ori- point of origin for DNA replication araC- gene for repressor protein in arabinose operon araB, A and D- gene for arabinose metabolism ara B, ara A & ara D

13. pGLO plasmid (engineered form) Gene for arabinose metabolism (ara BAD) has been replaced with GFP. Amipicillin resistance has been added (bla)