1. Biological Science to Working FRAMES 2.0 Model Workshop U.S. Nuclear Regulatory Commission Rockville, Maryland Pacific Northwest National Laboratory November 15-17, 2005

2. 2 Biologically Based Modeling VRTPBPK VCell Diesel Engine Gas Engine Coal Power Plant Sources of PM Emissions (Burning of Fossil Fuels) Atmosphere Soil Water Environmental Fate & Transport Atmospheric Transformations Atmospheric Chemistry Biological Receptor (Sensitive Populations) Biological Modeling Core Outcomes Analysis Biological Modeling FRAMES focuses on Source-to-Dose-to- Biological models, and their interrelationships Biological models developed and linked within FRAMES

3. 3 Lab-level Research and Development Question: Can the FRAMES integration method be compatible with modern System-Biology scientific knowledge? Simulation is common but not with tools like FORTRAN but with tools like MatLab, Mathematica, Maple, and SciLab. Why Scilab? Free for non-commercial use and as powerful as MatLab.

4. 4 The Model is from a Publication CL Tran, M Graham, D Bucahanon (2000) Institute of Occupational Medicine 8 Roxburgh Place, Edinburgh, EH8 9SU, UK 44(0) 131 667 5131 publications@iomhq.org.uk sent to JGT 03/05 via email

5. 5

6. A couple of command will plot one of the state variables from the system of equations

7. The plots produced from a couple of simple commands for plots

8. 8 To connect to FRAMES we need to either: 1)provide file that can be written by FRAMES, 2) have FRAMES write values to the script directly using tools like BPP, or 3) have a library set written for the specific tool to facilitate use in the environment (equivalent to a language specific interface). For the purpose of this demonstration we use #1. We still need at least some Java code to make it work.

9. Now the module can be used in tandem with an air source, and a lung deposition model from CIIT. The output of the lung deposition is written to the input of cell model.