1. Locating the Repressor Binding Sites in the Bacteriophages Presented by Sonia Sharma BNFO 301

2. CONFIDENTIAL2 Repressors What are They Bacteriophages when infecting the Bacteria have to make an important decision

3. CONFIDENTIAL 3 If Life is bestowed Lytic promoters have to be silenced Repressors come to limelight to do their job They Block the promoter’s sites for lytic pathway then proceed to Lysogeny Repressor also binds to DNA at specific sequences Found multiple times in genome

4. CONFIDENTIAL4 Objective is to locate those sites By Utilizing computational tools integrated in BioBIKE,- A graphical programming interface that enables biologist to approach complex research questions Upstream sequences of relevant phages

5. CONFIDENTIAL5 Data- available for Che12, e-lambda,L5 and Bxb1

6. CONFIDENTIAL6

7. 7 Same method for L5 and Bxb1

8. CONFIDENTIAL8

9. 9

10. Testing Time Apply the same technique to find to binding sites of some random phages Packman Shaka CONFIDENTIAL10

11. CONFIDENTIAL11

12. CONFIDENTIAL12 Concluding thought Results yet to be analyzed