Presentation on theme: "WP3WP3 State of the Art May 2004 CNR IAMC - Istituto per lAmbiente Marino Costiero Messina, Italy Università degli Studi di Messina CNR IAMC - Istituto."— Presentation transcript:
WP3WP3 State of the Art May 2004 CNR IAMC - Istituto per lAmbiente Marino Costiero Messina, Italy Università degli Studi di Messina CNR IAMC - Istituto per lAmbiente Marino Costiero Messina, Italy Università degli Studi di Messina
16 rRNA Distribution at Family level In study: >830 sequences analysed 9 levels for Urania from 3.8% of Salinity to BB 7 Levels for Bannockfrom 3.9% to 28% of salinity 8 Levels for Discoveryfrom 6.2% of salinity to BB 7 Levels for LAtalantefrom 4% of salinity to BB 1st Statistical aproach:Family level (-aceae) 2nd Statistical aproach: Division Level
Diversity indexes (families) High diversity – Low dominanceLow diversity, High dominance
7. Urania show a regular increasing of diversity along the gradient Family every basin seems to be a different world! 1. At level of bacterial Family every basin seems to be a different world! 3. Urania, Bannock, and LAtalante shows the highest diversity 4. Urania, Bannock, and LAtalante shows the lowest dominance 5. Discovery shows lower level of diversity 6. Discovery shows dominance of specific families at medium salinity concentration along the gradient 2. There is a grouping made by SW and BB layer………. Real o Bias?
1. There are a specific DHABs Configuration 2. There are some unique and sporadic phylotypes (probably contamination?) 3. There are Specific direct correlations within tipical DHABs flora. 4. Gamma proteobacteria shows to be often inverse related with typical DHABs Flora 5. Discovery Basin show a peculiar configuration with two big groups(KB1 and Desulfobatcereaceae) domainig two different layer of the basin interface 6. In Discovery and Bannock Basin KB1 and Verrucomicrobiales go very well related along the basin interface gradient 8. In Atalante, there are very well defined bacterial distributions
functional group: At level of functional group: 1. There is a common significance that join the more saline Urania layers with the Bannock and Atalante basins 2. Discovery maintains its peculiar bizarre world 3. Some layers of Urania seems to drop out from the common distribution
Proposed metabolic pathways in the DHABs 3380dbar e-e- e-e- e-e- e-e- e-e- e-e- e-e- e-e- e-e- Volatile fatty acids CO 2 Mn ++ Ba ++ SulphateSulphideSulphur Oxic Anoxic Acetate RedOx Potential NO 3 MnO 2 (FeOH) 3 SO 4 2- CO 2 O2O2O2O2 Eh:-232 -313 * Evidence of in situ active cells or functional genes involved in the pathways e-e- e-e- CO 2 CH 4 Methanogens * AOM and Methanohalophilus 16SrRNA SO 4 2- S-S- S0S0 SRB e-e- e-e- * dsrAB func.genes Fe(OH) 3 Fe 2+ FeRB e-e- * Geobacteriaceae 16SrRNA MnO 2 Mn 2+ Ba ++ Manganese oxidisers * Arcobacter 16SrRNA
Discovery Functional charachterisation In study: Distribution along the Discovery salinity graident of: Anaerobic Methane Oxidation related (ANME) bacteria (rRNA) Desulfobatcteraceae distribution (16rRNA) DSR Subunit A gene (RNA) DSR Subunit B gene (RNA)
1. At 6% of salinity of Discovery basin we found only members related to crenoarcheota division 2. At 11% and 19% the new DHVE3 3. The anaerobic methanophiles was found at 11% and 19% of salinity - Bacteria 4. 16SrRNA from 6% and 11% of salinity shows mainly Desulfobacteraceae related bacteria 5. dsrA and dsrB distribution shows two levels of diversity (macro and micro) 6. the dsr clusters related to the Desulfobacteriaceae show an high ratio of microdiversity with two defined sub-clusters: i) 6% of salinity clones ii) 6% and 11% togeter - Archaea
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