1. Molecular Axon Guidance PC12 cell…. then NGF added NGF is neurotropic and neurotrophic

2. Growth FactorsGuidance Factors

4. Netrin..was one of the first neurotropic factors

5. Findings

6. Netrin from FP

7. Netrin from FP, But perpendicular

8. Netrin in media

10. Cell locomotion like GCs Cell moves forward by extending filipodia & lamellipodia Focal adhesions are formed Cell is pulled forward

14. DiffusibleContact dependent

17. Extracellular matrix

18. Proteins of ECM - collagen

19. Extracellular matrix Proteins of ECM - proteoglycans

20. Extracellular matrix Proteins of ECM - fibronectin

21. Extracellular matrix Proteins of ECM - laminin collagen laminin

22. Interaction of cells with ECM inactive INTEGRINS

23. Interaction of cells with ECM INTEGRINS functions of outside-in signals differentiation motility growth survival ligands have RGD motif

24. Interaction of cells with ECM FOCAL ADHESIONS FA

25. Interaction of cells with ECM FOCAL ADHESION S

26. Interaction of cells with cells CELL ADHESION MOLECULES

27. Interaction of cells with ECM and other cells CELL ADHESION MOLECULES VS ECM ATTACHMENT MOLECULES

30. Tcf/Lef P E-Cadherin  -catenin p120 P Wnt Dsh Frz II Extracellular Space actin filaments  -catenin Ca 2+ Gsk3  APC Axin

31. Focus is often on adhesive function: Cadherins as molecular glue Cadherins as Master Regulators of Morphology Cadherins Actin Cytoskeleton  -catenin Rho-GTPases Arp2/3 Proliferation CDKI: p27/p21 Wnt Motility Passive Inhibition Rho-GTPases Active Promoter Adhesion AJ’s Type of Cadherin Stabilize others junctions Receptor Signaling EGF FGF PDGF Transcriptional/ Nuclear Role  -catenin/Wnt P120/kaiso In nucleus