2. AP Biology 2007-2008 Immune / Lymphatic System lymphocytes attacking cancer cell phagocytic leukocyte lymph system Fighting the Enemy Within!

3. AP Biology Avenues of attack  Points of entry  digestive system  respiratory system  urogenital tract  break in skin  Routes of attack  circulatory system  lymph system

4. AP Biology Why an immune system?  Attack from outside  lots of organisms want you for lunch!  animals are a tasty nutrient- & vitamin-packed meal  cells are packages of macromolecules  animals must defend themselves against invaders (pathogens)  viruses  HIV, flu, cold, measles, chicken pox  bacteria  pneumonia, meningitis, tuberculosis Lyme disease  fungi  yeast (“Athlete’s foot”…)  protists  amoeba, malaria  Attack from inside  cancers = abnormal body cells Mmmmm, What’s in your lunchbox?

5. AP Biology Lymph system Production & transport of leukocytes Traps foreign invaders lymph node lymph vessels (intertwined amongst blood vessels)

6. AP Biology Development of Red & White blood cells short-lived phagocytes 60-70% WBC develop into macrophages inflammatory response fight parasites Red blood cells Lymphocytes Leukocytes

7. AP Biology Lines of defense  1st line: ______________________  broad, external defense  “walls & moats”  skin & mucous membranes  2nd line: _____________________  broad, internal defense  “patrolling soldiers”  _____________________________  3rd line: _____________________  specific, acquired immunity  “elite trained units”  ________________________  ________________________ Bacteria & insects inherit resistance. Vertebrates acquire immunity.

8. AP Biology 1st line: Non-specific External defense  Barrier  skin  Traps  mucous membranes, cilia, hair, earwax  Elimination  coughing, sneezing, urination, diarrhea  Unfavorable pH  stomach acid, sweat, saliva, urine  Lysozyme enzyme  digests bacterial cell walls  tears, sweat Lining of trachea: ciliated cells & mucus secreting cells

9. AP Biology 2nd line: Non-specific patrolling cells  Patrolling cells & proteins  attack pathogens, but don’t “remember” for next time  ________________________  phagocytic white blood cells  macrophages, neutrophils, natural killer cells  ________________________  proteins that destroy cells  ________________________  increase in body temp.  increase capillary permeability  attract macrophages yeast macrophage bacteria

10. AP Biology Leukocytes: Phagocytic WBCs  Attracted by chemical signals released by damaged cells  ingest pathogens  digest in lysosomes  Neutrophils  most abundant WBC (~70%)  ~ 3 day lifespan  Macrophages  “big eater”, long-lived  Natural Killer Cells  destroy virus-infected cells & cancer cells

11. AP Biology  Natural Killer Cells perforate cells  release perforin protein  insert into membrane of target cell  forms pore allowing fluid to flow in & out of cell  cell ruptures (lysis)  ________________ Destroying cells gone bad! perforin punctures cell membrane cell membrane natural killer cell cell membrane virus-infected cell vesicle perforin

12. AP Biology Anti-microbial proteins  _________________________________  ~20 proteins circulating in blood plasma  attack bacterial & fungal cells  form a membrane attack complex  perforate target cell  apoptosis  cell lysis plasma membrane of invading microbe complement proteins form cellular lesion extracellular fluid complement proteins bacterial cell

13. AP Biology Inflammatory response  Damage to tissue triggers local non-specific inflammatory response  release chemical signals  histamines & prostaglandins  capillaries dilate, become more permeable (leaky)  delivers macrophages, RBCs, platelets, clotting factors  fight pathogens  clot formation  increases temperature  decrease bacterial growth  stimulates phagocytosis  speeds up repair of tissues

14. AP Biology Fever  When a local response is not enough  system-wide response to infection  activated macrophages release interleukin-1  triggers hypothalamus in brain to readjust body thermostat to raise body temperature  higher temperature helps defense  inhibits bacterial growth  stimulates phagocytosis  speeds up repair of tissues  causes liver & spleen to store iron, reducing blood iron levels  bacteria need large amounts of iron to grow

15. AP Biology  Specific defense with memory  _______________________  __________________  _______________________  immunoglobulins  Responds to…  ____________________  cellular name tags  specific pathogens  specific toxins  abnormal body cells (cancer) 3rd line: Acquired (active) Immunity B cell

16. AP Biology “self”“foreign” How are invaders recognized?  ________________________  cellular name tag proteins  “self” antigens  no response from WBCs  “foreign” antigens  response from WBCs  pathogens: viruses, bacteria, protozoa, parasitic worms, fungi, toxins  non-pathogens: cancer cells, transplanted tissue, pollen

17. AP Biology Lymphocytes  _____________________  mature in bone marrow  humoral response system  “humors” = body fluids  attack pathogens still circulating in blood & lymph  _______________________  _____________________  mature in thymus  cellular response system  attack invaded cells  “Maturation”  learn to distinguish “self” from “non-self” antigens  if react to “self” antigens, cells are destroyed during maturation bone marrow

18. AP Biology B cells  Attack, learn & remember pathogens circulating in blood & lymph  Produce specific antibodies against specific antigen  Types of B cells  ____________________  immediate production of antibodies  rapid response, short term release  ____________________  continued circulation in body  long term immunity

19. AP Biology Antibodies  ____________________________________  multi-chain proteins  binding region matches molecular shape of antigens  each antibody is unique & specific  millions of antibodies respond to millions of foreign antigens  tagging “handcuffs”  “this is foreign…gotcha!” each B cell has ~50,000 antibodies Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y antigen antigen- binding site on antibody variable binding region

20. AP Biology s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s s Structure of antibodies light chains antigen-binding site heavy chains antigen-binding site light chain B cell membrane heavy chains light chain variable region antigen-binding site Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y

21. AP Biology What do antibodies do to invaders? macrophage eating tagged invaders invading pathogens tagged with antibodies Y Y Y Y Y Y neutralizecaptureprecipitateapoptosis

22. AP Biology  Immunoglobulins  IgM  1st immune response  activate complement proteins  IgG  2nd response, major antibody circulating in plasma  promote phagocytosis by macrophages  IgA  in external secretions, sweat & mother’s milk  IgE  promote release of histamine & lots of bodily fluids  evolved as reaction to parasites  triggers allergic reaction  IgD  receptors of B cells??? macrophage eating tagged invaders invading pathogens tagged with antibodies Y Y Y Y Y Y Classes of antibodies Weeks 0246 IgMIgG Exposure to antigen Antibody levels

23. AP Biology macrophage B cell immune response ___________ (in blood & lymph) 10 to 17 days for full response invader (foreign antigen) ___________________ Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y ________________ Y Y Y Y Y Y Y Y ___________ 1000s of clone cells Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y ______________ _______________ ______________ _______________ Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y YY Y Y captured invaders Y Y Y Y Y Y Y Y Y Y Y Y Y

24. AP Biology Vaccinations  Immune system exposed to harmless version of pathogen  ___________________________________ ___________________________________  “active immunity”  rapid response on future exposure  creates immunity without getting disease!  Most successful against viruses

25. AP Biology Jonas Salk  Developed first vaccine  against polio  attacks motor neurons 1914 – 1995 April 12, 1955 Albert Sabin 1962 oral vaccine

26. AP Biology Polio epidemics 1994: Americas polio free

27. AP Biology  Obtaining antibodies from another individual  maternal immunity  antibodies pass from mother to baby across placenta or in mother’s milk  critical role of breastfeeding in infant health  mother is creating antibodies against pathogens baby is being exposed to  Injection  injection of antibodies  short-term immunity Passive immunity

28. AP Biology 2007-2008 What if the attacker gets past the B cells in the blood & actually infects (hides in) some of your cells? You need trained assassins to recognize & kill off these infected cells! T Attack of the Killer T cells! But how do T cells know someone is hiding in there?

29. AP Biology How are cells tagged with antigens?  ___________________________________________  proteins which constantly carry bits of cellular material from the cytosol to the cell surface  “snapshot” of what is going on inside cell  give the surface of cells a unique label or “fingerprint” T or B cell MHC protein MHC proteins displaying self-antigens Who goes there? self or foreign?

30. AP Biology How do T cells know a cell is infected?  Infected cells digest some pathogens  MHC proteins carry pieces to cell surface  foreign antigens now on cell membrane  called ____________________________________  macrophages can also serve as APC  tested by Helper T cells MHC proteins displaying foreign antigens infected cell T cell with antigen receptors T H cell WANTED

31. AP Biology T cells  Attack, learn & remember pathogens hiding in infected cells  recognize antigen fragments  also defend against “non-self” body cells  cancer & transplant cells  Types of T cells  ________________________  alerts rest of immune system  ________________________  attack infected body cells  ________________________  long term immunity T cell attacking cancer cell

32. AP Biology T cell response ____________ ____________ ____________ Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y YY Y Y killer T cell ____________ or interleukin 1 interleukin 2 helper T cell ___________ ________ ____________ APC: activated macrophage APC: infected cell

33. AP Biology Attack of the Killer T cells Killer T cell binds to infected cell  Destroys infected body cells  binds to target cell  secretes perforin protein  punctures cell membrane of infected cell  apoptosis infected cell destroyed cell membrane Killer T cell cell membrane target cell vesicle perforin punctures cell membrane

34. AP Biology Blood type blood type antigen on RBC antibodies in blood donation status A ___________ antigens on surface of RBC ___________ antibodies __ B ___________ antigens on surface of RBC ___________ antibodies __ AB ___________________ antigens on surface of RBC ______ antibodies_____________ O ________________ on surface of RBC ________________ antibodies_____________ Matching compatible blood groups is critical for blood transfusions A person produces antibodies against foreign blood antigens

35. AP Biology Immune response free antigens in bloodantigens on infected cells humoral responsecellular response Y Y Y Y YY Y Y Y Y Y skin pathogen invasion antigen exposure Y Y Y Y YY Y Y Y Y Y alert

36. AP Biology  Human Immunodeficiency Virus  _____________________________  helper T cells don’t activate rest of immune system: killer T cells & B cells  also destroys helper T cells  AIDS: Acquired ImmunoDeficiency Syndrome  infections by opportunistic diseases  death usually from “opportunistic” infections  pneumonia, cancers HIV & AIDS HIV infected T cell

37. AP Biology How to protect yourself…

38. AP Biology Immune system malfunctions  Auto-immune diseases  immune system attacks own molecules & cells  lupus  antibodies against many molecules released by normal breakdown of cells  rheumatoid arthritis  antibodies causing damage to cartilage & bone  diabetes  beta-islet cells of pancreas attacked & destroyed  multiple sclerosis  T cells attack myelin sheath of brain & spinal cord nerves  Allergies  over-reaction to environmental antigens  allergens = proteins on pollen, dust mites, in animal saliva  stimulates release of histamine

39. AP Biology 2009-2010 It’s safe to Ask Questions!