1. BELLRINGER 1. Write out the word for each chemical formula
NO3
NH3
NO2 N2
2. Why is the nitrogen cycle important to aquaponics?

2. Aquatic Nitrogen Cycle
The most important concept for Aquaponics!

3. Why is the aquatic nitrogen cycle important to aquaponics?
Arguably, the nitrogen cycle is the most important concept to be understood about aquaponics because, without it, there is no aquaponics.
The fish eat food and excrete organic waste matter in the form of urea, uric acid arising from the digestion of their food. They also emit ammonia as part of the gaseous exchange that occurs through respiration.
The ammonia compounds are toxic to fish and plants cannot absorb ammonia.
What would happen if the system was left this way?

4. Why is the aquatic nitrogen cycle important to aquaponics?
What allows the fish and plants to survive and thrive?
When ammonia levels in the fish tank reach a certain level, bacteria (Nitrosomonas) begin to colonize the system.
As the bacteria build up, the ammonia is converted to nitrite. As the ammonia levels dip, the nitrite levels increase.
The nitrites (like ammonia) are toxic to fish. 
Draw diagram on board as you go through this slide.

5. Why is the aquatic nitrogen cycle important to aquaponics?
When the nitrite levels reach a certain point, nitrite-oxidizing bacteria (nitrospira) colonize the system and convert the nitrites to nitrates.....which become plant food.
The plants take up the nitrates (and other compounds) and as they grow and are harvested, nitrogen is removed from the system.
Eventually, the system reaches the point where the various aspects of the nitrogen cycle happen simultaneously. As long as the fish are fed (and things remain in balance), the cycle continues.

6. Why do plants need Nitrogen?
Nitrogen is one of the main elements in protein. Nitrogen is also a component of nucleic acid, DNA, RNA, genes, chromosomes, enzymes, chlorophyll, secondary metabolites (alkaloids), and amino acids.
Protein is essential for all living organisms, and is required for growth and development.
Nitrogen accounts for about 1 to 6 % of plant dry matter, depending on the species and is often the limiting factor in plant growth.

7. Composition of Atmosphere
Nitrogen is the major gas in the atmosphere.
But it is often the limiting factor in plant growth
Why would nitrogen be limiting if it makes up most of the atmosphere?

8. Nitrogen Cycle
Unlike carbon or oxygen, nitrogen is not very available to life.
It’s conversion to a useable form requires biological activity
Cyclic conversions from one form to another are mainly mediated by bacteria.

9. Cycling of Nitrogen
Five processes participate in the cycling of nitrogen through the biosphere:
1) Nitrogen fixation
2) Decay
3) Nitrification
4) Denitrification
5)Dissimilation
Microorganisms play major roles in these processes

10. Process 1: Fixation N2  NH3
Nitrogen fixation refers to the conversion of nitrogen gas to either NH3 or NH4 by bacteria.
Terrestrial systems: soil bacteria in root nodules of legumes.
Aquatic systems: blue green algae.

11. Process 2: Nitrification
NH3  NO2  NO3
The term nitrification refers to the conversion of ammonium or ammonia to nitrate
Responsible: nitrifying bacteria known as chemoautotrophs.
These bacteria gain energy by converting NH3 or NH4 to NO2 or NO3
Plants take up NO3 to make proteins
while using CO2 as a source of carbon to synthesize organic compounds.

12. Process 3: Decay Proteins  NH3
Proteins pass through food webs just as carbohydrates do.
At each trophic level, organic nitrogen compounds are returned to the environment in waste excretions.
Final beneficiaries of these materials are microorganisms of decay.
They breakdown the molecules in excretions and dead organisms into ammonia (NH3).

13. Process 4: Denitrification
NO3  N2
By this process, NO3 in soil or water is converted into N2 gas.
This must occur under anaerobic conditions (anaerobic respiration).
Again, mediated by bacteria.
Should not happen in an aquaponics system!

14. Process 5: Dissimilation
NO3  NO2  NH3
By this process, nitrates in soil or water is converted back to nitrites and ammonia
This must occur under anaerobic conditions.
Again, mediated by bacteria.
Should not happen in an aquaponics system!

15. Simplified diagram of the nitrogen cycle that is established in an aquatic system

16. Aquatic Nitrogen Cycling
Conversion of ammonia (NH3) to nitrate (NO3-) is via chemoautotrophic bacteria.
First step (NH3  NO2) by Nitrosomonas sp.
second step(NO2  NO3) by Nitrospira sp.
Both steps/reactions use NH4+ and NO2- as an energy source, CO2 as a carbon source.
This is a non-photosynthetic type of growth.

17. Aquatic Nitrogen Cycling
Reaction runs best at pH 7-8 and 25-30oC.
However; under low Dissolved Oxygen, it runs in reverse.
NO3- is converted to NO2= and other forms.
Can go all the way backwards to NH3.

18. Ammonia Toxicity
Fish excrete ammonia (NH4). When you’re keeping fish at home or in an aquaponics lab it needs to be managed as it is very toxic to the fish. Decomposing food also creates ammonia, so don’t overfeed fish!
Some of the effects of excessive ammonia include:
Extensive damage to tissues, especially the gills and kidney
Impaired growth
Decreased resistance to disease
Death
Keep below 1 ppm
In a lake or ocean it’s all good because the vast volume of water dilutes this ammonia.

19. Nitrite Toxicity
Now, nitrite is much less poisonous to the fish than ammonia.
But it’s by not good either. It stops the fish from taking up oxygen.
Keep below 5 ppm

21. Today, we discussed Nitrogenous Compounds
Nitrogen cycle
Toxicity
Nitrogen Fixation
Decay
Nitrification
Denitrification
Nitrogen cycling
Nitrogen equilibria

22. Exit Slip-September 17, 2014 Explain the role of:
Nitrosomonas sp.
Nitrospira sp.
Draw a cycle/picture that includes:
Fish, waste, ammonia, nitrite, nitrate, plants, Nitrosomonas sp and nitrospira sp.