1.
Draw the nontemplate strand of DNA for the template shown below. Compare and contrast its base sequence with the mRNA molecule.
DNA A C C A A A C C G A G T
mRNA U G G U U U G G C U C A

2.
Draw the nontemplate strand of DNA for the template shown below. Compare and contrast its base sequence with the mRNA molecule.
DNA A C C A A A C C G A G T
T G G T T T G G C T C A
mRNA U G G U U U G G C U C A

3. 5’-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-3’
17.1 2.
What protein product would you expect from a poly-G mRNA that is 30 nucleotides long?
5’-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-3’

4. 5’-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-3’
17.1 2.
What protein product would you expect from a poly-G mRNA that is 30 nucleotides long?
5’-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-3’
Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly

5. 17.2 1.
Compare and contrast the functioning of DNA polymerase and RNA polymerase.

6. DNA polymerase
RNA polymerase

7. DNA polymerase
Assembles chains from monomers
RNA polymerase
Assembles chains from monomers

8. DNA polymerase
Assembles chains from monomers
Complementary base pairing
RNA polymerase
Assembles chains from monomers
Complementary base pairing

9. DNA polymerase
Assembles chains from monomers
Complementary base pairing
Reads 3’→5’
Assembles 5’ →3’
RNA polymerase
Assembles chains from monomers
Complementary base pairing
Reads 3’→5’
Assembles 5’ →3’

10. DNA polymerase
Needs a primer
RNA polymerase
Can start from scratch

11. DNA polymerase
Needs a primer to start
Uses A, T, G & C
Uses nucleotides containing deoxyribose
RNA polymerase
Can start from scratch
Uses A, U, G & C
Uses nucleotides containing ribose

12. 17.2 2.
Is the promoter at the upstream or downstream end of a transcription unit?

13. 17.2 2.
Is the promoter at the upstream or downstream end of a transcription unit?
Upstream

14. 17.2 3.
In a prokaryote, how does RNA polymerase “know” where to start transcribing a gene? In a eukaryote?

15. 17.2 3.
In a prokaryote, how does RNA polymerase “know” where to start transcribing a gene? In a eukaryote?
Prokaryote: RNA polymerase recognizes promoter
Eukaryote: Transcription factors mediate binding

16. 17.2 4.
How is the primary transcript produced by a prokaryotic cell different from that produced by a eukaryotic cell?

17. 17.2 4.
How is the primary transcript produced by a prokaryotic cell different from that produced by a eukaryotic cell?
Prokaryote: used immediately as mRNA
Eukaryote: Must be modified before being used as mRNA

18. 17.3 1.
How does the alteration of the 5’ and 3’ ends of pre-mRNA affect the mRNA that exists in the nucleus?

19. 17.3 1.
How does the alteration of the 5’ and 3’ ends of pre-mRNA affect the mRNA that exists in the nucleus?
Facilitates transportation
Prevents degradation
Facilitates ribosomal attachment

20. 17.3 2.
Describe the role of snRNPs in RNA splicing.

21. 17.3 2. Describe the role of snRNPs in RNA splicing.
Joins with other proteins to form spliceosomes.
Removes introns, splices exons together.

22. 17.3 3.
How can alternative RNA splicing generate a greater number of polypeptide products than there are genes?

23. 17.3 3.
How can alternative RNA splicing generate a greater number of polypeptide products than there are genes?
THE CAT ATE THE RBAT

24. 17.3 3.
How can alternative RNA splicing generate a greater number of polypeptide products than there are genes?
THE CAT ATE THE RBAT

25. 17.3 3.
How can alternative RNA splicing generate a greater number of polypeptide products than there are genes?
THE CAT ATE THE RBAT

26. 17.4 1.
Which two processes ensure that the correct amino acid is added to a growing polypeptide chain?

27. 17.4 1.
Which two processes ensure that the correct amino acid is added to a growing polypeptide chain?
Aminoacyl-tRNA synthase
tRNA codon

28. 17.4 2.
Describe how the formation of polyribosomes can benefit the cell.

29. 17.4 2.
Describe how the formation of polyribosomes can benefit the cell.
Multiple copies of a protein in a short time.

30. 17.4 3.
Describe how a polypeptide to be secreted is transported to the endomembrane system.
Signal peptide is recognized by SRP.
SPR brings polypeptide to ER lumen.

31. 17.5 1.
Describe three properties of RNA that allow it to perform diverse roles in the cell.
Hydrogen bonds with DNA or RNA
Specific 3-D shape
Catalize chemical reactions.

32. 17.6 1.
In figure (orange book and green book) number the RNA polymerases in order of their initiation of transcription. Then number each mRNA’s ribosomes in order of their initiation of translation.

33. 17.6 2.
Would the arrangement shown in Figure be found in a eukaryotic cell? Explain.

34. 17.7 1.
What happens when one nucleotide pair is lost from the middle of the coding sequence of a gene?

35. 17.7 1.
What happens when one nucleotide pair is lost from the middle of the coding sequence of a gene?
Frame shift mutation
Nonfunctional protein

36.
The template strand of a gene contains the sequence 3’-TACTTGTCCGATATC-5’. Draw a double strand of DNA and the resulting strand of mRNA, labeling all 5’ and 3’ ends. Determine the amino acid sequence. Then show the same after a mutation changes the template DNA sequence to 3’-TACTTGTCCAATATC-5’. What is the effect on the amino acid sequence?

37. 17.7 2. Template strand: 3’-TACTTGTCCGATATC-5’
Template strand:
3’-TACTTGTCCGATATC-5’
Draw a double strand of DNA.

38. 17.7 2. Double strand: 3’-TACTTGTCCGATATC-5’ 5’-ATGAACAGGCTATAG-3’
Double strand:
3’-TACTTGTCCGATATC-5’
5’-ATGAACAGGCTATAG-3’
Draw the resulting strand of mRNA, labeling all 5’ and 3’ ends

39. 17.7 2. Double strand: 3’-TACTTGTCCGATATC-5’ 5’-ATGAACAGGCTATAG-3’
Double strand:
3’-TACTTGTCCGATATC-5’
5’-ATGAACAGGCTATAG-3’
mRNA:
5’-AUGAACAGGCUAUAG-3’

40.
mRNA:
5’-AUGAACAGGCUAUAG-3’
Determine the amino acid sequence.

41.
mRNA:
AUG AAC AGG CUA UAG
Determine the amino acid sequence.

42.
mRNA:
AUG AAC AGG CUA UAG
Polypeptide:
Met-Asn-Arg-Leu

43.
Then show the same after a mutation changes the template DNA sequence to 3’-TACTTGTCCAATATC-5’.

44. 17.7 2. Template strand: 3’-TACTTGTCCAATATC-5’
Template strand:
3’-TACTTGTCCAATATC-5’
Draw a double strand of DNA.

45. 17.7 2. Double strand: 3’-TACTTGTCCAATATC-5’ 5’-ATGAACAGGTTATAG-3’
Double strand:
3’-TACTTGTCCAATATC-5’
5’-ATGAACAGGTTATAG-3’
Draw the resulting strand of mRNA, labeling all 5’ and 3’ ends

46. 17.7 2. Double strand: 3’-TACTTGTCCAATATC-5’ 5’-ATGAACAGGTTATAG-3’
Double strand:
3’-TACTTGTCCAATATC-5’
5’-ATGAACAGGTTATAG-3’
mRNA:
5’-AUGAACAGGUUAUAG-3’

47.
mRNA:
5’-AUGAACAGGUUAUAG-3’
Determine the amino acid sequence.

48.
mRNA:
AUG AAC AGG UUA UAG
Determine the amino acid sequence.

49.
mRNA:
AUG AAC AGG UUA UAG
Polypeptide:
Met-Asn-Arg-Leu

50. 17.7 2. mRNA: AUG AAC AGG UUA UAG Polypeptide: Met-Asn-Arg-Leu
mRNA:
AUG AAC AGG UUA UAG
Polypeptide:
Met-Asn-Arg-Leu
The resulting polypeptide is the same. .