1. Biotechnology BIT-110 (3 hrs)
Dr. Hajra Sadia (PhD, Biotechnology)

2. Course Outline Fundamentals of biotechnology
Core Techniques in Biotechnology
DNA Technology
From DNA to proteins
Principals of recombinant DNA technology
Microbial Biotechnology
Products from microorganisms
Fermentation: principal and application
Food biotechnology
Plant Biotechnology
Plant tissue culture and applications
Genetically Engineered Plants
Applications of Plant genetic engineering
Biofertilizers
Animal Biotechnology
Animal cell culture and characterization of cell lines
Application of animal cell culture
Transgenic animal technology
Environmental Biotechnology
Bioremediation
Phytoremediation
Utilization of biomass
Biomining and bioleaching
Biotechnology in medicine and health care
Gene therapy
Xenotransplantation
Forensic Biotechnology
Human Genome Project
Impacts of biotechnology on human beings: Biotechnology Ethics

3. Recommended Books Biotechnology Basic Biotechnology 3rd ed.
By Bourgaize et al. (2003)
Basic Biotechnology 3rd ed.
By Ratledge and Kristiansen (2008)
Biotechnology: Principles and Applications
By Rastogi (2007)
Biotechnology: An introduction
By Ignacimuthu (2008)

4. Marks distribution First 1 hr Exam 20 % Second 1 hr Exam 20 %
Assignment 5 %
Quiz 5 %
Terminal Exam 50 %

5. Biotechnology: An Introduction
What is Biotechnology?

6. What is Biotechnology ? bios = life teuchos = tool
logos = study of or essence of
e.g. the study of tools from living things/organisms
Classical definition
“Biotechnology is a set of tools that utilize living things (and more recently, derivatives of living things) to solve problems or to provide products”

7. What is Biotechnology ?
The application of various sciences (i.e., immunology, molecular biology, biochemistry, botany, animal science, etc.) to develop products or to solve problems.
Technology Assessment of the U.S. Congress defines biotechnology as
"any technique that uses living organisms or their products to make or modify a product, to improve plants or animals, or to develop microorganisms for specific uses."

8. What is Biotechnology ?
Utilization of a biological process, be it microbial, plant or animal cells or their constituents to provide goods and services to mankind.
Goods:
Products of industries concerned with food, beverages, pharmaceutical, biochemicals and winning of metals
Services:
Largely concerned with water purification, industrial and domestic waste management including sewage water

9. The Promise of Biotechnology
Diagnosing disease
Curing disease
Nutritious food/feed
Healthy food/feed
Productive land
Feeding the poor
Sustainable agriculture
Healthy environment

10. History of Biotechnology
Raw
Material
The term "biotechnology" was coined in 1919
by Karl Ereky, Hungarian engineer
All lines of work by which products are produced
from raw materials with the aid of living things
Upstream Processing
Fermentation and
Biotransformation
Downstream
Processing
Pure
Product

11. Traditional biotechnology has been used for thousands of years to produce improved food and health care products.
Modern biotechnology enables us to develop improved products more safely and more rapidly than ever before.
Biotechnology in one form or another has flourished since prehistoric times.

12. Examples…
They could plant their own crops and breed their own animals, they learned to use biotechnology.
The discovery that fruit juices fermented into wine, or that milk could be converted into cheese or yogurt, or that beer could be made by fermenting solutions of malt and hops began the study of Biotechnology
When the first bakers found that they could make a soft, spongy bread rather than a firm, thin cracker, they were acting as fledgling biotechnologists.
The first animal breeders, realizing that different physical traits could be either magnified or lost by mating appropriate pairs of animals, engaged in the manipulations of biotechnology.

13. Modern Biotechnology Recombinant genetic engineering…
…….Using biological process to develop products
G. Steven Burrill 1997

14. Why Biotechnology?
Bioproduction of drugs so complex they can only be synthesized in a living system

15. Origins of Biotechnology
Historical pharmaceutical biotechnology:
Alexander Fleming discovery of penicillin from bread mold
Large scale broth tank production of penicillin for injuries – Florey & Chain
Modern pharmaceutical biotechnology:
interspecies genetic transplantation
hybridoma – tumor cell and leukocyte fusions
heterologous protein production (microbes, animal and plant cells)

17. Biotechnology Timeline
1750 B.C. Sumerians use yeast to brew beer
500 B.C. Chinese use mold as an antibiotic to treat boils
1863 Mendel discovers transmission of genetic traits
1906 First early study of genes; term “genetics” introduced
1919 Term “biotechnology” first used by agriculturalist
1928 Penicillin discovered
1953 Watson and Crick discover double-helix structure of DNA
1960 First synthetic antibiotic

18. Biotechnology Timeline (Cont…)
The Green Revolution (dwarf wheat with 70% increased yield through hybridization)
1965 Mouse-human cells successfully fused
Genetic code cracked
1971 First complete synthesis of a gene
1971 Restriction enzyme discovered (EcoRI)
Recombinant DNA technology to cut and paste genes
1975 DNA sequencing
1975 Hybridoma technology (monoclonal antibodies)
1978 Insulin gene cloned
Live organism patented (Engineered mice)

19. Biotechnology: last 30 Years
1981 First transgenic animal
1982 First biotech drug marketed (Insulin)
1983 First transgenic plant (tobacco)
1983 First artificial chromosome
1985 Genetically engineered plants field tested
1986 Use of microbes to clean up oil spill
1986 Polymerase chain reaction by Kary Mullis, revolutionize molecular biology (Nobel prize 93)
1988 First patent for genetically altered animal (transgenic mouse)
1995 First non-viral full gene sequence completed

20. Biotechnology: last 20 Years (Cont…)
First mammal cloned (Dolly the cloned sheep unveiled)
2000 First entire plant genome sequenced (Arabidopsis thaliana)
2001 Cloning banned (the US house of representatives passes the human cloning prohibition act of 2001, a ban on all human cloning
2002 Mapping of human genome virtually complete
2002-date Rethinking of RNA (scientists are forced to rethink their view of RNA when they discover how important small pieces of RNA are in controlling many cell functions
2005 Human genome confirmed

21. BIOTECHNOLOGY Crops Drugs Vaccines Diagnostic Livestock Environment
Molecular
Biology
Microbiology
Biochemistry
Genetics
Chemical
Engineering
Cell
Biology
BIOTECHNOLOGY
Crops
Drugs
Vaccines
Diagnostic
Livestock
Environment

22. Biotechnology
Biotechnology is an integrated use of biochemistry, microbiology and engineering sciences to achieve technological applications of the capabilities of microbes, cultured tissue cells and parts thereof for production of value added products.

23. Applications
Biotechnology has applications in four major industrial areas, including
health care (medical),
crop production and agriculture,
non food (industrial) uses of crops and other products (e.g. biodegradable plastics, vegetable oil, biofuels), and
environmental uses.

24. Branches of Biotechnology

25. Bioinformatics
An interdisciplinary field which addresses biological problems using computational techniques, and makes the rapid organization and analysis of biological data possible.
The field may also be referred to as computational biology,
conceptualizing biology in terms of molecules and then applying informatics techniques to understand and organize the information associated with these molecules, on a large scale.
Bioinformatics plays a key role in various areas, such as functional genomics, structural genomics, and proteomics, and forms a key component in the biotechnology and pharmaceutical sector.

26. Blue biotechnology
Blue biotechnology is concerned with the application of molecular biological methods to marine and freshwater organisms.
It involves the use of these organisms, and their derivatives, for purposes such as increasing seafood supply and safety, controlling the proliferation of noxious water-borne organisms, and developing new drugs.

27. Green biotechnology Biotechnology applied to agricultural processes.
Selection and domestication of plants via micropropagation.
Designing of transgenic plants to grow under specific environments in the presence (or absence) of chemicals.
Green biotechnology might produce more environmentally friendly solutions than traditional industrial agriculture.
Engineering of a plant to express a pesticide, thereby ending the need of external application of pesticides.
E.g. Bt corn.

28. Red biotechnology
application of biotechnology in the medicine and human healthcare sector.
Therapeutic proteins
Designing of organisms to produce antibiotics, and
Engineering of genetic cures through genetic manipulation.

29. White biotechnology
Also known as industrial biotechnology, is biotechnology applied to industrial processes.
Designing of an organism to produce a useful chemical.
Use of enzymes as industrial catalysts to either produce valuable chemicals or destroy hazardous/polluting chemicals.
White biotechnology tends to consume less in resources than traditional processes used to produce industrial goods.

30. Applications of Biotechnology:
Food Industry
Pharmaceutical Industry
Chemical Industry
Enzyme Industry
Mining Industry
Agricultural Industry
Environmental Industry

32. THANKS