Presentation is loading. Please wait.

Presentation is loading. Please wait.

Jiangsu Institute of Nuclear Medicine Contact:

Published byBrooks Gautney Modified over 9 years ago

Similar presentations

Presentation on theme: "Jiangsu Institute of Nuclear Medicine Contact:"— Presentation transcript:

1 Jiangsu Institute of Nuclear Medicine Contact: zhangli@jsinm.org
10th AOTA Congress BALI, INDONESIA, 2012 Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu, China Contact:

2 Curcumin ? An acitve polyphenol derived from the plant turmeric
low toxicity anti-inflammatory antioxidant antiangiogenic antitumour Curcumin Thyroid cancer ? Chemical structure of curcumin

3 Apoptosis and Autophagy
Apoptosis –type I programmed cell death Autophagy- type II programmed cell death - a self-degradative process - cope with stresses - required in apoptotic-deficient cells Three types of autophgay: Microautophagy Chaperone-mediated autophagy Macroautopahgy

4 Part I Crucumin suppresses BCPAP cells growth

5 Effects of curcumin on cell viability

6 Effects of curcumin on cell morphology

7 PI/Hoechst staining A B

8 Part II Curcumin induces apoptosis in BCPAP cells

9 Effects of curcumin on pro-apoptotic and anti-apoptotic proteins
B

10 Effects of curcumin on loss of mitochondrial membrane potential (MMP)

11 Caspase-dependent apoptosis in curcumin-treated cells

12 Part III Curcumin induces autophagy in BCPAP cells

13 Induction of autophagy in curcumin-treated cells
B The FASEB JournalJune 2003vol. 17 no. 9 

14 Induction of autophagy in curcumin-treated cells
RFP-LC3 Curcumin (mM) 50 C

15 Effects of curcumin on the phosphorylation of AKT pathway
B

16 Effects of curcumin on the phosphorylation of MAP kinases

17 Curcumin selectively induces autopagy in tumor cells
B

18 Curcumin seletively induces cancer cell death
B

19 Dynamic distribution of curcumin in BCPAP cells
Enlarged 6 h 12 h 24 h

20 Effects of curcumin on DNA conformation
B

21 Conclusions Curcumin induces cytotoxicity of BCPAP cells.
Induction of  apoptosis  and autophagy as the likely underlying mechanism that is responsible for the cytotoxicity of curcumin . Curcumin inhibited AKT pathway and activated mitogen-activated protein kinase (MAPK) signaling thereby inducing autophagy.

22 Thank you for your attention!
Acknowledgements Jiangsu Institute of Nuclear Medicine Grants Bao Jiandong Yu Huixin Zhang Chiyu Wu Jing Zheng Jie Sun Wei China Medical University Guan Haixia Nangjing University Sun Yang National Natural Science Foundation of China (No ) Natural Science Foundation of Jiangsu Province (No. BK ) Science and Research Foundation of Health Bureau of Jiangsu Province (No. H ) Thank you for your attention!

Download ppt "Jiangsu Institute of Nuclear Medicine Contact:"

Similar presentations

Tianhong Pan, MD, PhD; Wenjie Xie, MD; Pawan Rawal,MD

Tianhong Pan, MD, PhD; Wenjie Xie, MD; Pawan Rawal,MD
Cancer and the Cell Cycle : An overview Ken Wu. Disclaimer This tutorial is a simple and conceptual guide to the cancer module and the cell cycle If there.

Cancer and the Cell Cycle : An overview Ken Wu. Disclaimer This tutorial is a simple and conceptual guide to the cancer module and the cell cycle If there.
Apoptosis By Douglas R. Green

Apoptosis By Douglas R. Green
Fundamentals of Cell Biology Chapter 13: The Birth and Death of Cells

Fundamentals of Cell Biology Chapter 13: The Birth and Death of Cells
P53, Apoptosis, Cancer, More Regulation G1 checkpoint Controlled by G1 Cdk-cyclin G1 cyclin levels also vary with the cell cycle Many additional levels.

P53, Apoptosis, Cancer, More Regulation G1 checkpoint Controlled by G1 Cdk-cyclin G1 cyclin levels also vary with the cell cycle Many additional levels.
Timosaponin A3 is a steroidal saponin from Anemarrhena asphodeloides that has a selective cytotoxic activity towards cancer cells Frank King, Sylvia Fong,

Timosaponin A3 is a steroidal saponin from Anemarrhena asphodeloides that has a selective cytotoxic activity towards cancer cells Frank King, Sylvia Fong,
Apoptosis By Dr Abiodun Mark .A.

Apoptosis By Dr Abiodun Mark .A.
ApoptosisNecrosis Apoptosis is a form of programmed cell death Apoptosis is responsible for the formation of digits in the developing mouse paw. Apoptotic.

ApoptosisNecrosis Apoptosis is a form of programmed cell death Apoptosis is responsible for the formation of digits in the developing mouse paw. Apoptotic.
ER stress is partially responsible for BN108- and TspA3- induced apoptosis C. D. E. F. Figure 3. A. Western blot analysis of the levels of phosphorylated.

ER stress is partially responsible for BN108- and TspA3- induced apoptosis C. D. E. F. Figure 3. A. Western blot analysis of the levels of phosphorylated.
Effects of NGF. Developmental processes during which apoptosis can occur.

Effects of NGF. Developmental processes during which apoptosis can occur.
Chlamydiae Obligate intracellular pathogens. Obligate intracellular pathogens. Acute and/or persistent infections. Acute and/or persistent infections.

Chlamydiae Obligate intracellular pathogens. Obligate intracellular pathogens. Acute and/or persistent infections. Acute and/or persistent infections.
What is apoptosis?? Apoptosis is a morphological term

What is apoptosis?? Apoptosis is a morphological term
Cell Division and Apoptosis III Chapter 17. Activation of a cyclin dependent kinase.

Cell Division and Apoptosis III Chapter 17. Activation of a cyclin dependent kinase.
Research on Gastric Cancer treatment by TCM Zhao Hailei, Associate Dean, International Education College, Shanghai University of Traditional Chinese Medicine,

Research on Gastric Cancer treatment by TCM Zhao Hailei, Associate Dean, International Education College, Shanghai University of Traditional Chinese Medicine,
Bcl-2 family There are three classes of Bcl-2 according to their domains and apoptotic effect: – Anti-apoptotic proteins contain four specific domains.

Bcl-2 family There are three classes of Bcl-2 according to their domains and apoptotic effect: – Anti-apoptotic proteins contain four specific domains.
Apoptosis, controlled cell death, is a natural process of development. Recently it has been found that mitochondria play an important role in apoptosis.

Apoptosis, controlled cell death, is a natural process of development. Recently it has been found that mitochondria play an important role in apoptosis.
Apoptosis – mechanisms and role in cancer therapy

Apoptosis – mechanisms and role in cancer therapy
Apoptosis (Programmed Cell Death). Apoptosis vs Necrosis Level of stress, change in environment stress apoptosisnecrosis.

Apoptosis (Programmed Cell Death). Apoptosis vs Necrosis Level of stress, change in environment stress apoptosisnecrosis.
APOPTOSIS Pathway of cell death in which cells activate enzymes that degrade the cells’ own nuclear DNA and nuclear and cytoplasmic proteins.

APOPTOSIS Pathway of cell death in which cells activate enzymes that degrade the cells’ own nuclear DNA and nuclear and cytoplasmic proteins.
Isosteviol derivatives induced apoptosis in Human lung cancer via targeting MEK/MAPK pathway: An in vitro and in vivo study Ahmed M Malki 1,,PhD Stephen.

Isosteviol derivatives induced apoptosis in Human lung cancer via targeting MEK/MAPK pathway: An in vitro and in vivo study Ahmed M Malki 1,,PhD Stephen.

Similar presentations

Ads by Google