1. How to interpret enzyme graphs
ENZYME ACTIVITY
How to interpret enzyme graphs
(NEXT
SLIDE)

2. Reading Graphs
Which graph shows the amount of substrate going to zero faster?
Substrate
Substrate
Time (sec)
Time (sec)

3. TRY AGAIN
So…
Nope, the amount of substrate gets to zero in less time in the OTHER graph.
Substrate
Substrate
Time (sec)
Time (sec)

4. RIGHT!!
So…
YES!! The amount of substrate gets to zero in less time in this graph.
Substrate
Substrate
Time (sec)
Time (sec)

5. So… Which enzyme works FASTER? Substrate Substrate Time (sec)

6. TRY AGAIN
So…
Nope, not this one. The faster the enzyme works, the faster the substrate will decrease.
Time (sec)
Substrate
Substrate
Time (sec)

7. RIGHT!!
So…
YES!! This enzyme works faster because the substrate decreases faster!!
Time (sec)
Substrate
Substrate
Time (sec)

8. What do you think can affect the speed of an enzyme?
TEMPERATURE pH
AMOUNT OF
LIGHT

9. What do you think can affect the speed of an enzyme?
TEMPERATURE
RIGHT!! Changing the pH of the reaction can affect the speed of an enzyme.
ANYTHING ELSE? pH
AMOUNT OF
LIGHT

10. What do you think can affect the speed of an enzyme?
TEMPERATURE
RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme. pH
AMOUNT OF
LIGHT

11. What do you think can affect the speed of an enzyme?
TEMPERATURE
RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme.
ANYTHING ELSE? pH
AMOUNT OF
LIGHT

12. What do you think can affect the speed of an enzyme?
TEMPERATURE
RIGHT!! Changing the pH of the reaction can ALSO affect the speed of an enzyme. pH
AMOUNT OF
LIGHT

13. What do you think can affect the speed of an enzyme?
TEMPERATURE
Sorry, most enzymes are NOT affected by the amount of light…
Pick again pH
AMOUNT OF
LIGHT

14. Factors affecting enzyme speed
We can make a graph of enzyme speed at different temperatures.

15. Graph of ENZYME SPEED What should go on the X axis? Speed of enzyme
Temperature (°C)

16. Graph of ENZYME SPEED TRY AGAIN
Nope, the speed of the enzyme is what we are MEASURING,it should go on the Y axis.
Speed of enzyme
Temperature (°C)

17. RIGHT! Temperature should go on the X axis.
Graph of ENZYME SPEED
Amount of substrate
What should go on the Y axis?
(HINT: This is what we are
MEASURING)
Speed of enzyme
RIGHT! Temperature should go on the X axis. 10 20 30 40 50 60
Temperature (°C)

18. Graph of ENZYME SPEED TRY AGAIN Amount of substrate
What should go on the Y axis?
(HINT: This is what we are
MEASURING)
We’re not measuring the amount of substrate this time.
Speed of enzyme 10 20 30 40 50 60
Temperature (°C)

19. REMEMBER!! The enzyme is working FASTER
Graph of ENZYME SPEED
FAST
RIGHT!!
The speed of enzyme should go on the Y axis.
Speed of enzyme
REMEMBER!! The enzyme is working FASTER
As you go UP the Y axis.
SLOW 10 20 30 40 50 60
Temperature (°C)

20. Let’s GRAPH!!
FAST
Amylase is found in saliva. Click on the red button that is approximately at body temperature
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

21. Let’s GRAPH!! TRY AGAIN Amylase is found in saliva.
FAST
Amylase is found in saliva.
5°C is close to freezing.
TOO COLD!!
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

22. Let’s GRAPH!! TRY AGAIN Amylase is found in saliva.
FAST
Amylase is found in saliva.
22°C is close to room temperature.
TOO COLD!!
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

23. Let’s GRAPH!! TRY AGAIN Amylase is found in saliva. 55°C is 131°F.
FAST
Amylase is found in saliva.
55°C is 131°F.
TOO HOT!!
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

24. Let’s GRAPH!! Amylase is found in saliva. 37°C is body temperature.
FAST
Amylase is found in saliva.
37°C is body temperature.
RIGHT!! Amylase will work best at about 37°C because it is normally found in your mouth
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

25. At very low temperatures
Let’s GRAPH!!
FAST
Amylase works slowly
At very low temperatures
Because it is not close
To body temperature
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

26. Let’s GRAPH!! Amylase will work Faster as it gets Closer to body
Temperature.
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

27. Let’s GRAPH!! Amylase will work fastest at body Temperature. FAST
Speed of enzyme
The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE
SLOW 10 20 30 40 50 60
Temperature (°C)

28. As the temperature gets too hot, Amylase doesn’t work as well.
Let’s GRAPH!!
As the temperature gets too hot,
Amylase doesn’t work as well.
FAST
Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

29. Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE FAST Speed of enzyme
SLOW 10 20 30 40 50 60
Temperature (°C)

30. Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE RIGHT!! FAST
Speed of enzyme
RIGHT!!
SLOW 10 20 30 40 50 60
Temperature (°C)

31. What is the optimum temperature for this enzyme?
Temperature (°C) 20 50 60 10 40 30
Speed of enzyme
SLOW
FAST
10°C
20°C
30°C

32. What is the optimum temperature for this enzyme?
TRY AGAIN
What is the optimum temperature for this enzyme?
Temperature (C) 20 50 60 10 40 30
Speed of enzyme
SLOW
FAST
REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at.
10°C
20°C
30°C

33. What is the optimum temperature for this enzyme?
TRY AGAIN
What is the optimum temperature for this enzyme?
Temperature (°C) 20 50 60 10 40 30
Speed of enzyme
SLOW
FAST
REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at.
10°C
20°C
30°C

34. RIGHT!!
Right!!

35. What is the optimum pH for this enzyme?
FAST 9
Speed of enzyme 10 11
SLOW 2 4 6 8 10 12 pH

36. What is the optimum pH for this enzyme?
TRY AGAIN
What is the optimum pH for this enzyme?
FAST
Look closely… 9
Speed of enzyme 10 11
SLOW 2 4 6 8 10 12 pH

37. What is the optimum pH for this enzyme?
TRY AGAIN
What is the optimum pH for this enzyme?
FAST
Look closely… 9
Speed of enzyme 10 11
SLOW 2 4 6 8 10 12 pH

38. RIGHT!!
Right!!

39. What is the optimum temperature for this enzyme?
FAST
20°C
Speed of enzyme
63°C
48°C
SLOW 20 30 40 50 60 70 80
Temperature (°C)

40. What is the optimum temperature for this enzyme?
TRY AGAIN
What is the optimum temperature for this enzyme?
FAST
20°C
Speed of enzyme
63°C
48°C
SLOW 20 30 40 50 60 70 80
Temperature (°C)

41. What is the optimum temperature for this enzyme?
TRY AGAIN
What is the optimum temperature for this enzyme?
FAST
20°C
Speed of enzyme
63°C
48°C
SLOW 20 30 40 50 60 70 80
Temperature (°C)

42. RIGHT!!
Right!!
You rock at this...

43. What temperature does the enzyme work best at?
Time
Substrate
Temperature =
25°C
Time
Substrate
Temperature =
30°C
Time
Substrate
Temperature =
35°C
At 25°C
At 30°C
At 35°C

44. REMEMBER: the faster the substrate decreases, the faster the enzyme is working
Time
Substrate
Temperature =
25°C
Time
Substrate
Temperature =
30°C
Time
Substrate
Temperature =
35°C
At 25°C
At 30°C
At 35°C

45. REMEMBER: the faster the substrate decreases, the faster the enzyme is working
Time
Substrate
Temperature =
25°C
Time
Substrate
Temperature =
30°C
Time
Substrate
Temperature =
35°C
At 25°C
At 30°C
At 35°C

46. TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ
RIGHT!!
TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ

47. QUIZ - QUESTION 1 SALIVA LIVER STOMACH
Where in your body is the amylase enzyme found?
SALIVA
LIVER
STOMACH

48. QUIZ - QUESTION 1 TRY AGAIN SALIVA LIVER STOMACH
Where in your body is the amylase enzyme found?
SALIVA
LIVER
STOMACH

49. QUIZ - QUESTION 1 TRY AGAIN SALIVA LIVER STOMACH
Where in your body is the amylase enzyme found?
SALIVA
LIVER
STOMACH

50. RIGHT!!
Right!!

51. QUIZ - QUESTION 2 Click on the CATALYST in this reaction: SUGAR STARCH
AMYLASE

52. QUIZ - QUESTION 2 TRY AGAIN
REMEMBER: The catalyst performs the reaction, but isn’t used up
SUGAR
STARCH
AMYLASE

53. QUIZ - QUESTION 2 TRY AGAIN
REMEMBER: The catalyst performs the reaction, but isn’t used up
SUGAR
STARCH
AMYLASE

54. RIGHT!!
Right!!

55. QUIZ - QUESTION 3
Click on the substrate that is most likely to react with the enzyme below:
STARCH
PROTEIN
LIPID

56. QUIZ - QUESTION 3 TRY AGAIN
REMEMBER: The subtrate that fits into a protein will be the one that reacts with it.
STARCH
PROTEIN
LIPID

57. QUIZ - QUESTION 3 TRY AGAIN
REMEMBER: The subtrate that fits into a protein will be the one that reacts with it.
STARCH
PROTEIN
LIPID

58. RIGHT!!
Right!!
PROTEIN

59. QUIZ - QUESTION 4
What is the most likely name of the enzyme in question 3 that reacts with protein?
PROTEINOSE
PROTEINATION
PROTEINZYME
PROTEINASE

60. QUIZ - QUESTION 4 TRY AGAIN
NOPE: sugars end in -OSE, not enzymes (sucrose, lactose, glucose)
PROTEINOSE
PROTEINATION
PROTEINZYME
PROTEINASE

61. QUIZ - QUESTION 4 TRY AGAIN NOPE: think about “lactase” and “amylase”
PROTEINOSE
PROTEINATION
PROTEINZYME
PROTEINASE

62. QUIZ - QUESTION 4 TRY AGAIN NOPE: think about “lactase” and “amylase”
PROTEINOSE
PROTEINATION
PROTEINZYME
PROTEINASE

63. RIGHT!!
Right!!
PROTEINASE

64. QUIZ - QUESTION 5
Click on the part of the graph that shows the OPTIMAL temperature for this enzyme
Enzyme Activity
Temperature (°C)

65. QUIZ - QUESTION 5 TRY AGAIN
REMEMBER: the OPTIMAL temperature for an enzyme is the temperature where the enzyme works BEST (highest activity)
Enzyme Activity
Temperature (°C)

66. RIGHT!!
Right!!

67. QUIZ - QUESTION 6
Click on the graph that shows the optimum pH for this enzyme.
pH = 5.0
pH = 7.0
pH = 3.0
Substrate
Substrate
Substrate
Time
Time
Time

68. QUIZ - QUESTION 6 TRY AGAIN
REMEMBER: Substrate decreases in LESS time if the enzyme works faster
pH = 5.0
pH = 7.0
pH = 3.0
Substrate
Substrate
Substrate
Time
Time
Time

69. QUIZ - QUESTION 6 TRY AGAIN
REMEMBER: Substrate decreases in LESS time if the enzyme works faster
pH = 5.0
pH = 7.0
pH = 3.0
Substrate
Substrate
Substrate
Time
Time
Time

70. RIGHT!!
Right!!

71. RIGHT!!
Right!!

72. You have successfully completed the enzyme powerpoint.
CONGRATULATIONS!